Kiliç Abdullah, Senses Zeynep, Aydoğan Hakan, Başustaoglu Ahmet
GATA Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dall, Etlik, Ankara.
Mikrobiyol Bul. 2006 Oct;40(4):295-9.
Enterococcus species are the inhabitants of gastrointestinal flora and can cause endocarditis, urinary tract infections, and bacteremia. In recent years, infections caused by vancomycin-resistant enterococci (VRE) have increased in our country. The infections caused by VRE are treated with glycopeptide antibiotics like vancomycin and teicoplanin. Although seven different resistant genes have been described in VRE, VanA is the most frequently detected one in Turkey. The aim of this study was (i) to genotype four vancomycin-resistant Enterococcus faecium strains isolated in our hospital, by using pulsed field gel electrophoresis (PFGE); and (ii) to detect the type of Van gene by using polymerase chain reaction. Three of the strains were isolated from blood cultures and one from cerebrospinal fluid of the hospitalized patients between the years 2000-2004, and identified by both conventional methods and commercial kits. By using PFGE, we detected that isolates were different according to Tenover criteria and they had VanA genes. As a result, VanA carrying VRE cause sporadic infections in our hospital. The molecular epidemiologic investigation of resistant microorganisms is important in terms of infection control and epidemiology.
肠球菌是胃肠道菌群的成员，可引起心内膜炎、尿路感染和菌血症。近年来，我国耐万古霉素肠球菌（VRE）引起的感染有所增加。VRE引起的感染用万古霉素和替考拉宁等糖肽类抗生素治疗。尽管在VRE中已描述了七种不同的耐药基因，但VanA是土耳其最常检测到的一种。本研究的目的是：（i）使用脉冲场凝胶电泳（PFGE）对我院分离的四株耐万古霉素屎肠球菌菌株进行基因分型；（ii）使用聚合酶链反应检测Van基因的类型。其中三株菌株是在2000年至2004年间从住院患者的血培养物中分离出来的，一株是从脑脊液中分离出来的，并通过传统方法和商业试剂盒进行了鉴定。通过PFGE，我们检测到分离株根据Tenover标准不同，且它们具有VanA基因。结果，携带VanA的VRE在我院引起散发性感染。耐药微生物的分子流行病学调查在感染控制和流行病学方面很重要。
