Panzer Simon, Eichelberger Beate, Koren Daniela, Kaufmann Karin, Male Christoph
Clinic for Blood Group Serology and Department of Pediatrics, Medical University Vienna, Vienna, Austria.
Transfusion. 2007 Jan;47(1):103-6. doi: 10.1111/j.1537-2995.2007.01070.x.
Bernard-Soulier syndrome (BSS) patients may repeatedly require transfusion of platelets (PLTs). The hemostatic competence of transfused PLTs requires monitoring.
Flow cytometry and a cone and plate(let) analyzer (Impact-R, DiaMed) were used to monitor survival and function of transfused PLTs in a 7-year-old girl with BSS undergoing surgery. Flow cytometry was applied to differentiate autologous PLTs from transfused PLTs by staining for CD42b. The Impact, which measures PLT adhesion and aggregation in response to high shear stress, was used to evaluate PLT function.
Transfused PLTs were detectable by flow cytometry for 1 week after transfusion. While the patient's PLTs did not respond to high shear stress before transfusion, a normal response was documented by the Impact on the day after transfusion and 1 week thereafter.
Transfused PLTs were detectable by flow cytometry, and their functional activity was demonstrated by the Impact.
伯纳德-索利尔综合征（BSS）患者可能反复需要输注血小板（PLTs）。输注的血小板的止血能力需要监测。
采用流式细胞术和锥板分析仪（Impact-R，DiaMed）监测一名接受手术的7岁BSS女孩输注血小板的存活情况和功能。通过对CD42b进行染色，应用流式细胞术区分自体血小板和输注的血小板。Impact用于测量血小板在高剪切应力下的黏附和聚集情况，以评估血小板功能。
输注后1周内，通过流式细胞术可检测到输注的血小板。输血前患者的血小板对高剪切应力无反应，但输血后第1天及之后1周，Impact记录到正常反应。
通过流式细胞术可检测到输注的血小板，且其功能活性可通过Impact得到证实。
