Takanashi M, Urabe A, Nakayama J, Hori Y
Department of Dermatology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Dermatologica. 1991;183(3):187-90. doi: 10.1159/000247667.
Using immunohistochemical methods, we investigated the distribution of epithelial membrane antigen (EMA) on the normal eccrine gland, eccrine poroma and hidroacanthoma simplex. Granular membrane-associated reaction of EMA was detected on the outer cells of both the intraepidermal and the upper portion of intradermal eccrine ducts, as well as on the luminal surfaces and intercellular canaliculi of eccrine glands. Clear immunolabeling was also present in the tumor cells of eccrine poroma and hidroacanthoma simplex. Thus, it is suggested that the constituent cells of these tumors originate from the outer cells of the intraepidermal and/or the upper portion of the intradermal eccrine ducts. There was no immunolabeling for EMA on the tumor cells of seborrheic keratosis and basal cell carcinoma. Immunohistochemical staining for EMA is a useful tool for the diagnosis of skin appendage tumors.
我们采用免疫组织化学方法，研究了上皮膜抗原（EMA）在正常小汗腺、小汗腺汗孔瘤和单纯性汗腺棘皮瘤上的分布情况。在表皮内和真皮内小汗腺导管上部的外层细胞上，以及小汗腺的管腔表面和细胞间小管中，均检测到EMA的颗粒状膜相关反应。在小汗腺汗孔瘤和单纯性汗腺棘皮瘤的肿瘤细胞中也出现了清晰的免疫标记。因此，提示这些肿瘤的组成细胞起源于表皮内和/或真皮内小汗腺导管上部的外层细胞。脂溢性角化病和基底细胞癌的肿瘤细胞上未出现EMA免疫标记。EMA免疫组织化学染色是诊断皮肤附属器肿瘤的一种有用工具。
