博来霉素抗性作为真核生物盘基网柄菌转化的选择标记。

Bleomycin resistance as a selectable marker for transformation of the eukaryote, Dictyostelium discoideum.

作者信息

Chang A C, Hall R M, Williams K L

机构信息

School of Biological Sciences, Macquarie University, Sydney, N.S.W., Australia.

出版信息

Gene. 1991 Oct 30;107(1):165-70. doi: 10.1016/0378-1119(91)90312-y.

DOI:10.1016/0378-1119(91)90312-y

PMID:1720754

Abstract

An expression cassette was constructed, which has the bacterial bleomycin (Bm) resistance-encoding gene (ble) fused to the Dictyostelium discoideum actin-6 promoter, with a segment of 3'-flanking DNA from the actin-8-encoding gene placed downstream from the ble gene to serve as a transcription terminator. Plasmid pMUW161, which contains this cassette and the D. discoideum plasmid Ddp2 origin of DNA replication, transformed D. discoideum with high efficiency under Bm selection. Hence, this construct is useful as a dominant selectable marker for D. discoideum.

摘要

构建了一个表达盒，其具有与盘基网柄菌肌动蛋白-6启动子融合的细菌博来霉素（Bm）抗性编码基因（ble），并将来自肌动蛋白-8编码基因的一段3'侧翼DNA置于ble基因下游作为转录终止子。含有该表达盒和盘基网柄菌质粒Ddp2 DNA复制起点的质粒pMUW161，在Bm选择下能高效转化盘基网柄菌。因此，该构建体可作为盘基网柄菌的显性选择标记。