台湾格雷夫斯病患者的细胞因子启动子多态性
Cytokine promoter polymorphisms in Taiwanese patients with Graves' disease.
作者信息
Shiau Ming-Yuh, Huang Chien-Ning, Yang Tzi-Peng, Hwang Yi-Ching, Tsai Kan-Jen, Chi Chieh-Ju, Chang Yih-Hsin
机构信息
Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan, Republic of China.
出版信息
Clin Biochem. 2007 Feb;40(3-4):213-7. doi: 10.1016/j.clinbiochem.2006.11.009. Epub 2006 Dec 5.
OBJECTIVES
This study aimed to examine the association between promoter polymorphisms of Th1 and Th2 cytokine genes [interleukin-4 (IL-4 T-34C, A-81G, C-285T and T-589C), IL-6 (G-174C), IL-10 (A-592C and T-819C) and tumour necrosis factor-alpha (TNF-alpha G-238A and G-308A)] and Graves' disease (GD) in Taiwanese population.
DESIGN AND METHODS
Genomic DNA was extracted from peripheral blood cells of 137 GD patients and 189 control subjects. Cytokine gene polymorphisms were analyzed by polymerase chain reaction and restriction fragment length polymorphism.
RESULTS
Genotype frequencies of TNF-alpha G-238A or G-308A between control and GD subjects were significantly different. Frequencies of the high TNF-alpha secreting alleles (-238A and -308A) and IL-10 -819*C allele were significantly increased in GD patients. No significant differences regarding IL-4 or IL-6 gene polymorphisms between GD patients and control subjects were found.
CONCLUSIONS
Our data demonstrated that TNF-alpha G-238A and G-308A genotypes were strongly associated with GD incidence.
目的
本研究旨在探讨台湾人群中Th1和Th2细胞因子基因[白细胞介素-4（IL-4 T-34C、A-81G、C-285T和T-589C）、IL-6（G-174C）、IL-10（A-592C和T-819C）以及肿瘤坏死因子-α（TNF-α G-238A和G-308A）]启动子多态性与格雷夫斯病（GD）之间的关联。
设计与方法
从137例GD患者和189例对照者的外周血细胞中提取基因组DNA。通过聚合酶链反应和限制性片段长度多态性分析细胞因子基因多态性。
结果
对照者与GD患者之间TNF-α G-238A或G-308A的基因型频率存在显著差异。GD患者中高分泌TNF-α的等位基因（-238A和-308A）以及IL-10 -819*C等位基因的频率显著增加。未发现GD患者与对照者在IL-4或IL-6基因多态性方面存在显著差异。
结论
我们的数据表明，TNF-α G-238A和G-308A基因型与GD发病率密切相关。
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