Lundberg Kristina, Josefsson Ann, Nordin Conny
Department of Neuroscience and Locomotion, Psychiatry Section, Faculty of Health Sciences, Linköping University, SE-581 85 Linköping, Sweden.
Neuropeptides. 2007 Feb;41(1):59-63. doi: 10.1016/j.npep.2006.09.049. Epub 2007 Jan 8.
Cholecystokinin (CCK) was determined in plasma obtained from 10 female (aged 23.4+/-SD 2.3 years) and nine male (aged 22.0+/-SD 1.4 years) healthy volunteers. Blood samples were drawn three times (8.00a.m., 12 noon and 8.00a.m.) on each of two sessions, one in the winter (November-December) and one in the summer (April-July). The participants had fasted (and were nicotine-free) since midnight preceding the sampling. A standardized breakfast was served after the first sampling. CCK was determined by radioimmunoassay. The area under the curve 0-24h (AUC)(CCK Winter) was lower than AUC(CCK Summer) (F(1:17)=4.73; P=0.0440) in the whole group of volunteers. On comparing the CCK concentrations within each session, there was an overall difference in winter (F(2:36)=14.81; P<0.0001) as well in summer (F(2:36)=18.39; P<0.0001). Post hoc comparisons yielded a difference between the 8.00a.m. and 12 noon concentrations on the first day in winter (t=-3.96; P=0.0009) as well as in summer (t=-4.64; P=0.0002). The difference between the summer and winter AUCs(CCK) correlated with the difference between AUCs for temperatures in summer and winter (r=0.58; P=0.0089). The correlation was accounted for by the females (r=0.73; P=0.0171). The results are in accord with a diurnal and a seasonal variation of CCK in human plasma.
对10名女性（年龄23.4±标准差2.3岁）和9名男性（年龄22.0±标准差1.4岁）健康志愿者采集的血浆样本进行胆囊收缩素（CCK）测定。在两个时间段各采集三次血样（上午8点、中午12点和上午8点），一次在冬季（11月至12月），一次在夏季（4月至7月）。受试者在采样前一天午夜起禁食（且未接触尼古丁）。首次采样后提供标准化早餐。采用放射免疫分析法测定CCK。在全体志愿者中，0 - 24小时曲线下面积（AUC）（冬季CCK）低于AUC（夏季CCK）（F(1:17)=4.73；P = 0.0440）。比较每个时间段内的CCK浓度，冬季（F(2:36)=14.81；P < 0.0001）和夏季（F(2:36)=18.39；P < 0.0001）均存在总体差异。事后比较显示，冬季第一天上午8点和中午12点的浓度存在差异（t = -3.96；P = 0.0009），夏季也存在差异（t = -4.64；P = 0.0002）。夏季和冬季AUC（CCK）的差异与夏季和冬季温度AUC的差异相关（r = 0.58；P = 0.0089）。这种相关性在女性中更为明显（r = 0.73；P = 0.0171）。结果表明人体血浆中CCK存在昼夜和季节性变化。
