Kooistra A, König J J, Romijn J C, Schröder F H
Erasmus University/Dijkzigt Hospital, Department of Urology, Rotterdam, The Netherlands.
Anticancer Res. 1991 Jul-Aug;11(4):1495-500.
The influence of prostatic fibroblasts on the growth of the prostatic carcinoma cell lines PC-3 and LNCaP was examined. In a double layer soft agar system, clonal growth of both cell lines was inhibited by all prostatic fibroblasts tested, irrespective of whether they were derived from malignant or non malignant prostate tissue. Irradiated fibroblasts and quiescent fibroblasts showed similar effects. The finding that growth was also inhibited in the presence of fibroblasts conditioned medium suggests that the observed effects were mediated by a diffusable growth inhibiting factor. Organ-specific production was suggested by the observation that skin fibroblasts stimulated rather than inhibited PC-3 cell growth. These findings indicate a negative control of epithelial cell proliferation by prostatic stroma.
研究了前列腺成纤维细胞对前列腺癌细胞系PC-3和LNCaP生长的影响。在双层软琼脂系统中，所有测试的前列腺成纤维细胞均抑制了这两种细胞系的克隆生长，无论它们是源自恶性还是非恶性前列腺组织。照射后的成纤维细胞和静止的成纤维细胞表现出相似的作用。在成纤维细胞条件培养基存在下生长也受到抑制这一发现表明，观察到的作用是由一种可扩散的生长抑制因子介导的。皮肤成纤维细胞刺激而非抑制PC-3细胞生长这一观察结果提示了器官特异性的产生。这些发现表明前列腺基质对上皮细胞增殖具有负调控作用。
