Lang S H, Stower M, Maitland N J
Department of Biology, University of York, Heslington, UK.
Br J Cancer. 2000 Feb;82(4):990-7. doi: 10.1054/bjoc.1999.1029.
To study the effects of stromal epithelial cell interactions on prostate cancer metastasis, we have used primary human prostatic stromal cells derived from malignant and non-malignant tissues and established epithelial cell lines from normal (PNT1a and PNT2-C2) and tumour (PC-3, DU145 and LNCaP) origins. The effects of stromal cells on epithelial cell growth were studied in direct and indirect (using culture inserts) co-culture and by exposure to stromal cell-conditioned medium (assessed by MTT assay). The influence of stromal cells on epithelial cell invasion was measured using matrigel invasion chambers and on epithelial cell motility using time lapse microscopy. Results indicated that epithelial cell line growth was similarly unaffected or inhibited by stromal cells derived from malignant (n = 8) or non-malignant tissue (n = 8). In contrast, PNT2-C2 and PC-3 cells were found to be the least and the most invasive and motile epithelia respectively. Stromal cultures enhanced the invasion of both epithelial cells, but no differences were observed between the use of malignant and non-malignant tissues. All stromal cultures modestly stimulated PNT2-C2 motility but displayed a greater stimulation of PC-3 cell motility, while stromal cells derived from malignant tissue stimulated PNT2-C2 and PC-3 cell motility more than stromal cultures from non-malignant tissues.
为了研究基质上皮细胞相互作用对前列腺癌转移的影响，我们使用了源自恶性和非恶性组织的原代人前列腺基质细胞，并建立了来自正常（PNT1a和PNT2 - C2）和肿瘤（PC - 3、DU145和LNCaP）来源的上皮细胞系。通过直接和间接（使用培养插入物）共培养以及暴露于基质细胞条件培养基（通过MTT法评估）来研究基质细胞对上皮细胞生长的影响。使用基质胶侵袭小室测量基质细胞对上皮细胞侵袭的影响，并使用延时显微镜测量对上皮细胞运动性的影响。结果表明，源自恶性（n = 8）或非恶性组织（n = 8）的基质细胞对上皮细胞系生长的影响相似，要么无影响，要么有抑制作用。相比之下，发现PNT2 - C2和PC - 3细胞分别是侵袭性和运动性最低和最高的上皮细胞。基质培养增强了两种上皮细胞的侵袭能力，但在使用恶性和非恶性组织之间未观察到差异。所有基质培养均适度刺激了PNT2 - C2的运动性，但对PC - 3细胞运动性的刺激更大，而源自恶性组织的基质细胞比源自非恶性组织的基质培养对PNT2 - C2和PC - 3细胞运动性的刺激更大。
