通过最近邻识别测量证明的流体双层中胆固醇 - 磷脂络合作用
Cholesterol-phospholipid complexation in fluid bilayers as evidenced by nearest-neighbor recognition measurements.
作者信息
Zhang Jianbing, Cao Honghua, Regen Steven L
机构信息
Department of Chemistry, Lehigh University, Bethlehem, Pennsylvania 18015, USA.
出版信息
Langmuir. 2007 Jan 16;23(2):405-7. doi: 10.1021/la062950p.
Nearest-neighbor recognition experiments have been carried out using varying ratios of exchangeable dimer analogs of 1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol and 1,2-distearoyl-sn-glycero-3-phosphatidylglycerol in cholesterol-rich unilamellar vesicles at 60 degrees C. Equilibrium dimer distributions that were obtained support a structural model of the liquid-ordered bilayer in which free cholesterol and the longer-chain phospholipid homodimer are in equilibrium with a complex of unique stoichiometry, where one cholesterol molecule combines with two of the long-chain phospholipid homodimers. In this model, the mixing of the short-chain phospholipids with the uncomplexed long-chain phospholipids is ideal, and the complexed dimers are shielded from the disulfide exchange reaction.
在60摄氏度下，使用不同比例的1,2-二肉豆蔻酰-sn-甘油-3-磷脂酰甘油和1,2-二硬脂酰-sn-甘油-3-磷脂酰甘油的可交换二聚体类似物，在富含胆固醇的单层囊泡中进行了近邻识别实验。所获得的平衡二聚体分布支持了液相有序双层的结构模型，其中游离胆固醇和长链磷脂同型二聚体与具有独特化学计量比的复合物处于平衡状态，即一个胆固醇分子与两个长链磷脂同型二聚体结合。在该模型中，短链磷脂与未复合的长链磷脂的混合是理想的，并且复合二聚体免受二硫键交换反应的影响。
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