Shitan Nobukazu, Tanaka Miyako, Terai Koichiro, Ueda Kazumitsu, Yazaki Kazufumi
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Japan.
Biosci Biotechnol Biochem. 2007 Jan;71(1):242-5. doi: 10.1271/bbb.60441. Epub 2007 Jan 7.
To examine whether human ATP-binding cassette (ABC) transporters play a role in the detoxification of plant alkaloid berberine, we investigated berberine transport using multidrug resistance protein1 (MDR1) and multidrug resistance-associated protein1 (MRP1). Cells expressing MDR1 or MRP1 accumulated less berberine. Berberine accumulation depended on the cellular ATP level, and was reversed by typical inhibitors of MDR1, suggesting that human MDR1 and MRP1 directly efflux berberine as their substrate.
为了研究人类ATP结合盒（ABC）转运蛋白是否在植物生物碱黄连素的解毒过程中发挥作用，我们使用多药耐药蛋白1（MDR1）和多药耐药相关蛋白1（MRP1）研究了黄连素的转运。表达MDR1或MRP1的细胞积累的黄连素较少。黄连素的积累取决于细胞内的ATP水平，并被MDR1的典型抑制剂逆转，这表明人类MDR1和MRP1直接将黄连素作为底物进行外排。
