Kamoshida Shingo, Watanabe Kana, Suzuki Mai, Mizutani Yasuyoshi, Sakamoto Kazuki, Sugimoto Yoshikazu, Oka Toshinori, Fukushima Masakazu, Tsutsumi Yutaka
Department of Pathology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
Biomed Res. 2006 Dec;27(6):275-81. doi: 10.2220/biomedres.27.275.
We immunohistochemically evaluated the involvement of five cancer cachexia-related factors, including leukemia-inhibitory factor (LIF), zinc-alpha2-glycoprotein (ZAG), interleukin 6 (IL-6), proteolysis-inducing factor (PIF) and tumor necrosis factor alpha (TNF alpha) in causing cancer cachexia. Twenty-six xenografts implanted into mice were examined for the expression of the cancer cachexia-related factors, in relation to the body weight loss of the hosts. Five xenografts were categorized in the cachectic group, and the remaining 21 xenografts belonged to the non-cachectic group. LIF was extensively expressed in both the cachectic and non-cachectic groups. ZAG and IL-6 were expressed in one of the cachectic and some non-cachectic xenografts. PIF and TNF alpha were detected in one and two non-cachectic xenografts, respectively, but in none of the cachectic ones. Any of five factors examined were not conclusive for causing cancer cachexia in the murine xenograft model. Further analysis is needed in order to elucidate the mechanisms responsible for cancer cachexia.
我们采用免疫组化方法评估了包括白血病抑制因子（LIF）、锌α2糖蛋白（ZAG）、白细胞介素6（IL-6）、蛋白水解诱导因子（PIF）和肿瘤坏死因子α（TNFα）在内的五种癌症恶病质相关因子在引发癌症恶病质中的作用。检测了植入小鼠体内的26个异种移植物中癌症恶病质相关因子的表达情况，并将其与宿主的体重减轻情况进行关联分析。5个异种移植物被归类为恶病质组，其余21个异种移植物属于非恶病质组。LIF在恶病质组和非恶病质组中均广泛表达。ZAG和IL-6在部分恶病质异种移植物及一些非恶病质异种移植物中表达。PIF分别在1个非恶病质异种移植物中被检测到，TNFα在2个非恶病质异种移植物中被检测到，但在所有恶病质异种移植物中均未检测到。在小鼠异种移植模型中，所检测的这五种因子中的任何一种都不能确凿地证明其会引发癌症恶病质。为了阐明导致癌症恶病质的机制，还需要进一步分析。
Zotero 插件