Zhang X, Qiao J Y, Zhou X J, Yao F R, Wei Z C
Department of Immunology and Pathogenic Biology, Medical School of Xi'an Jiaotong University, P.O. Box 64, 205 Scarlet Bird Avenue, Xi'an, 710061, People's Republic of China.
Parasitol Res. 2007 Jun;101(1):43-51. doi: 10.1007/s00436-006-0439-x. Epub 2007 Jan 11.
The in vitro culture of Blastocystis hominis in Roswell Park Memorial Institute (RPMI) 1,640 medium containing 20% calf serum is described. The morphological and reproductive mode studies of B. hominis in symptomatic patients' faeces and in further RPMI 1,640 medium-cultured samples were undertaken by light microscopy using iodine staining and hematoxylin staining. Three distinct morphological forms, vacuolar, granular and amoeboid, were distinguished in faeces and in vitro cultures. The cystic form was detected in long-term cultures. Five modes of reproduction, namely, binary fission, endodyogeny, plasmotomy, budding and schizogeny were observed.
本文描述了人芽囊原虫在含有20%小牛血清的罗斯韦尔公园纪念研究所（RPMI）1640培养基中的体外培养。通过光学显微镜使用碘染色和苏木精染色，对有症状患者粪便中的人芽囊原虫以及在RPMI 1640培养基中进一步培养的样本进行形态学和繁殖方式研究。在粪便和体外培养物中区分出三种不同的形态形式，即空泡型、颗粒型和阿米巴型。在长期培养物中检测到了包囊形式。观察到五种繁殖方式，即二分裂、内二殖、质裂、出芽和裂体生殖。
