Wu Hsu-Shui, Lin Hui-Ting, Wang Chi-Kuang Leo, Chiang Yen-Feng, Chu Hsueh-Ping, Hu Meng-Chun
Graduate Institute of Physiology, National Taiwan University College of Medicine, Taipei, Taiwan, Republic of China.
Genesis. 2007 Feb;45(2):59-65. doi: 10.1002/dvg.20266.
The first step of steroid biosynthesis is catalyzed by cytochrome P450scc, encoded by CYP11A1. To achieve steroidogenic tissue-specific inactivation of genes in vivo by the Cre-loxP approach, we used the 4.4-kb regulatory region of the human CYP11A1 gene to drive Cre recombinase expression in the tissues that produce steroids. The resulting SCC-Cre mice express high levels of Cre in the adrenal cortex and gonads at the same sites as that for the endogenous CYP11A1 expression. In addition, Cre activity was found in the diencephalon and midbrain. In the developing brain, the Cre activity was first detected in the embryonic day 10.5. Our study is the first to show that the 4.4-kb CYP11A1 promoter is transcriptionally active in the brain in vivo.
类固醇生物合成的第一步由细胞色素P450scc催化，该酶由CYP11A1编码。为了通过Cre-loxP方法在体内实现类固醇生成组织特异性基因失活，我们使用人类CYP11A1基因的4.4 kb调控区域来驱动Cre重组酶在产生类固醇的组织中表达。由此产生的SCC-Cre小鼠在肾上腺皮质和性腺中与内源性CYP11A1表达相同的位点高水平表达Cre。此外，在间脑和中脑也发现了Cre活性。在发育中的大脑中，Cre活性最早在胚胎第10.5天被检测到。我们的研究首次表明4.4 kb的CYP11A1启动子在体内大脑中具有转录活性。
