Abdel Aal T M, Youssef F G, el-Hady H M, Sabry N M, Habib K S, Saber J M
Department of Parasitology, Faculty of Medicine, Ain Shams University.
J Egypt Public Health Assoc. 1996;71(3-4):321-41.
Three serological tests: Immunodiffusion (ID), Counterimmunoelectrophoresis (CIEP) and Enzyme-linked immunosorbent assay (ELISA) were used to study the role of crude adult worm antigen (CAWA) of Toxocara canis and each of its purified fractions in the serodiagnosis of human toxocariasis. Sensitivities of the three tests were lower in the ocular than in the visceral group, using different antigens. Purified fraction 1 showed more sensitive and specific reactions in the three tests, compared to CAWA or purified fraction 2 (P-F2) antigen. The other purified fractions (P-F3, P-F4 and P-F5) gave no positive reactions in any of the three tests. Using P-F1 antigen, ELISA was the most sensitive technique for diagnosis of both visceral and ocular toxocariasis followed by CIEP and then ID and the difference was statistically significant. However, CIEP was the most specific test followed by ELISA and lastly ID test. The ELISA test using Excretory-Secretory (E-S) larval antigen of Toxocara canis was less sensitive than the ELISA test using P-F1, although it was 100% specific. Thus, ELISA test using P-F1 is the test of choice for diagnosis of human toxocariasis, but when the specificity of a reaction is in doubt, CIEP test using the same antigen can be of value.
免疫扩散法（ID）、对流免疫电泳法（CIEP）和酶联免疫吸附测定法（ELISA），研究犬弓首蛔虫成虫粗抗原（CAWA）及其各纯化组分在人体弓首蛔虫病血清诊断中的作用。使用不同抗原时，三种检测方法在眼部组的敏感性均低于内脏组。与CAWA或纯化组分2（P-F2）抗原相比，纯化组分1在三种检测中表现出更敏感和特异的反应。其他纯化组分（P-F3、P-F4和P-F5）在三种检测中均未产生阳性反应。使用P-F1抗原时，ELISA是诊断内脏型和眼型弓首蛔虫病最敏感的技术，其次是CIEP，然后是ID，差异具有统计学意义。然而，CIEP是最特异的检测方法，其次是ELISA，最后是ID检测。使用犬弓首蛔虫排泄分泌（E-S）幼虫抗原的ELISA检测敏感性低于使用P-F1的ELISA检测，尽管其特异性为100%。因此，使用P-F1的ELISA检测是诊断人体弓首蛔虫病的首选检测方法，但当对反应的特异性存疑时，使用相同抗原的CIEP检测可能有价值。
