Vanerková Dana, Jandera Pavel, Hrabica Jaroslav
Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Nám. Cs. Legií 565, CZ-532 10 Pardubice, Czech Republic.
J Chromatogr A. 2007 Mar 2;1143(1-2):112-20. doi: 10.1016/j.chroma.2006.12.075. Epub 2006 Dec 23.
Ion-pairing reversed-phase high-performance liquid chromatography (RP-HPLC) operation conditions were studied to obtain information useful for development and optimization of separation methods suitable for HPLC/MS analysis of dyes. The retention of eight sulphonated azodyes with widely differing structures (1-5 acid groups, molecular weight range 350-1220) was measured in mobile phases containing various ion-pairing reagents in aqueous-methanolic mobile phases. The effects of the type and of the concentration of ammonium acetate, tetrabutylammonium hydrogen sulphate and five di- and tri-alkylammonium acetate ion-pairing reagents on the chromatographic behaviour of dyes were compared in mobile phases with varying concentrations of methanol. Structural effects on the retention of dyes were studied in detail. The retention scale based on lipophilic and polar indices can be used for optimization of mobile phase for HPLC/MS of dyes and, on the other hand, may provide some information on the structure of unknown dyes.
研究了离子对反相高效液相色谱（RP-HPLC）的操作条件，以获取有助于开发和优化适用于染料HPLC/MS分析的分离方法的有用信息。在含有各种离子对试剂的水-甲醇流动相中，测定了八种结构差异很大（含1-5个酸性基团，分子量范围为350-1220）的磺化偶氮染料的保留情况。在甲醇浓度不同的流动相中，比较了乙酸铵、硫酸氢四丁基铵和五种二烷基和三烷基乙酸铵离子对试剂的类型和浓度对染料色谱行为的影响。详细研究了染料结构对保留的影响。基于亲脂性和极性指数的保留标度可用于优化染料HPLC/MS的流动相，另一方面，可为未知染料的结构提供一些信息。
