[变形杆菌属和普罗威登斯菌属细菌的细胞内核苷酸聚合酶。其分离及特性]
[Intracellular nucleodepolymerase of bacteria, representatives of the Proteus and Providencia groups. Its isolation and properties].
作者信息
Sokolova R B, Beliaeva M I, Iusupova D V
出版信息
Vopr Med Khim. 1976 Jul-Aug;22(4):488-93.
High activity of enzymes, splitting native, denaturated DNA, deoxyribooligonucleotides and RNA, was observed in cell free extracts of bacteria--representatives of 5 strains of Proteus-Providencia. Some properties of nucleases were studied in cell free extracts. In the bacteria studied DNAases were thermolabile proteins, which were completely inactivated at 50-60 degrees, RNAases were more thermostable. The pH optima of the DNAases were at pH 9.0-11.0 in cell free system; RNAases were maximally active at pH 8.0-9.0
在属于变形杆菌-普罗威登斯菌属5个菌株代表的细菌无细胞提取物中，观察到了对天然、变性DNA、脱氧核糖寡核苷酸和RNA具有切割活性的高活性酶。对无细胞提取物中的核酸酶的一些特性进行了研究。在所研究的细菌中，DNA酶是热不稳定蛋白，在50-60摄氏度时会完全失活，RNA酶则更耐热。在无细胞体系中，DNA酶的最适pH值为9.0-11.0；RNA酶在pH 8.0-9.0时活性最高。
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