Tereshchenko S E, Riazanov S L, Zimin Iu I, Kotelevtsev Iu V
Lab Delo. 1991(9):54-6.
Cardiomyocyte membrane proteins (CMP) were isolated from the hearts of subjects dead from injuries or brain tumors by differentiated centrifugation in sucrose density gradient for the determination of anti-CMP antibodies in the blood serum samples of myocarditis patients. CMP preparation reactivity with blood serum antibodies of patients with myocarditis and other myocardial diseases, as well as with systemic lupus erythematosus, was assessed by the immune blotting technique. The findings evidence a high incidence of antibody interaction with CMP with a molecular mass of 67 kD. The method is highly specific and sensitive, its results are fairly well reproducible, and the technique is simple.
通过蔗糖密度梯度离心法从因外伤或脑肿瘤死亡的受试者心脏中分离出心肌细胞膜蛋白（CMP），用于测定心肌炎患者血清样本中的抗CMP抗体。采用免疫印迹技术评估CMP制剂与心肌炎及其他心肌疾病患者血清抗体以及系统性红斑狼疮患者血清抗体的反应性。研究结果表明，分子量为67 kD的CMP与抗体相互作用的发生率很高。该方法具有高度特异性和敏感性，结果具有良好的可重复性，且技术简单。
