Yu Yahong, Chen Zhong, Ye Qifa
Department of General Surgery, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2006;26(5):580-2. doi: 10.1007/s11596-006-0526-3.
In order to evaluate the applied value of soluble intracellular adhesion molecule-1 (sICAM-1) in acute rejection (AR) following liver transplantation, the expression of sICAM-1 protein was sequentially detected by using ELISA in serum and bile of 43 patients receiving liver transplantation. In AR group, the expression levels of sICAM-1 protein were increased 3 days before and immediately on the establishment of AR diagnosis, and there was significant difference in the expression of bile between AR group and control group (P<0.01). After reversion of AR with hormone intensive therapy, there was significant difference in the sICAM-1 protein expression of serum and bile between AR group and control group. It was concluded that the sequential detection of sICAM-1 protein level in serum and bile was a reliable and noninvasive method for the early diagnosis of AR after liver transplantation and was valuable to observe the curative effects of anti-AR therapy.
为评估可溶性细胞间黏附分子-1（sICAM-1）在肝移植术后急性排斥反应（AR）中的应用价值，采用酶联免疫吸附测定法（ELISA）对43例肝移植患者血清及胆汁中sICAM-1蛋白表达进行动态检测。AR组患者在AR诊断确立前3天及确诊时sICAM-1蛋白表达水平升高，AR组与对照组胆汁中sICAM-1蛋白表达差异有统计学意义（P<0.01）。经激素强化治疗AR逆转后，AR组与对照组血清及胆汁中sICAM-1蛋白表达差异有统计学意义。结论：动态检测血清及胆汁中sICAM-1蛋白水平是肝移植术后AR早期诊断的可靠无创方法，对观察抗AR治疗疗效有重要价值。
