Huang Qi, Chen Dayu, Fu Xiangning, Zu Yukun
Department of Thoracic and Cardiac Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2006;26(5):591-3. doi: 10.1007/s11596-006-0529-0.
The cell killing effects and bystander effects of double suicide gene on pulmonary carcinoma cells were explored. Lung adenocarcinoma cells (A549) were transfected with different titers of adenovirus vector and followed with different concentrations of 5-FC after a recombinant adenovirus vector carrying CD/UPRT gene (Ad-CD/UPRT) was constructed. The cell viability was measured by MTT assay 4 days later. The cell viability was dropped to 30.57 %-8.62 % after 10 MOI of Ad-CD/UPRT transfected and 5-FC (10-1000 microg/mL) administration. Furthermore, Ad-CD/UPRT-infected A549 cells showed a profound neighbor cell killing effect in the same methods. These results suggested that Ad-CD/UPRT/5-FC system can effectively suppress growth of lung adenocarcinoma cells, which may provide a novel and powerful candidate for lung cancer gene therapy strategies.
探讨双自杀基因对肺癌细胞的杀伤作用及旁观者效应。构建携带CD/UPRT基因的重组腺病毒载体（Ad-CD/UPRT）后，用不同滴度的腺病毒载体转染肺腺癌细胞（A549），并在转染后给予不同浓度的5-氟胞嘧啶（5-FC）。4天后通过MTT法检测细胞活力。用10MOI的Ad-CD/UPRT转染并给予5-FC（10 - 1000μg/mL）后，细胞活力降至30.57% - 8.62%。此外，采用相同方法，Ad-CD/UPRT感染的A549细胞对邻近细胞显示出显著的杀伤作用。这些结果表明，Ad-CD/UPRT/5-FC系统可有效抑制肺腺癌细胞的生长，这可能为肺癌基因治疗策略提供一种新的有力候选方法。
