Li Lu, Liu Jihong, Li Jiagui, Ye Zhangqun
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2006;26(5):607-9. doi: 10.1007/s11596-006-0534-3.
The types of the voltage-dependent calcium channels (VDCCs) in human ejaculatory sperm and the effects of calcium channel blocker (CCB) on human sperm motility parameters in vitro were investigated. The human sperm motility parameters in vitro in response to the pharmacological agents nifedipine (NIF, inhibitor of L-type VDCC) and co-conotoxin (GVIA, inhibitor of N-type VDCC) were compared and analyzed statistically. The results showed that NIF (1, 5, 10 micromol/L) could not only significantly affect human sperm's shape but also spermatozoa motility after incubated at least 10 min in vitro (P<0.001). GVIA (0.1, 0.5 and 1 micromol/L) could just only significantly affect human sperm's progressive motility (a %+b %) after incubated for 20 min in vitro (P<0.01), but they both could not significantly affect spermic abnormality rate. It is suggested that L-type VDCC, non L-type VDCCs and isoform of L-type VDCC exist in the cell membrane of human sperm solely or together, and they participate in the spermic physiological processes especially the spermic motility.
研究了人类射出精子中电压依赖性钙通道（VDCCs）的类型以及钙通道阻滞剂（CCB）对体外人类精子运动参数的影响。比较并统计分析了药物硝苯地平（NIF，L型VDCC抑制剂）和芋螺毒素（GVIA，N型VDCC抑制剂）对体外人类精子运动参数的影响。结果显示，NIF（1、5、10微摩尔/升）在体外孵育至少10分钟后，不仅能显著影响人类精子的形态，还能影响精子活力（P<0.001）。GVIA（0.1、0.5和1微摩尔/升）在体外孵育20分钟后仅能显著影响人类精子的前向运动（a%+b%）（P<0.01），但二者均不能显著影响精子畸形率。提示人类精子细胞膜上单独或共同存在L型VDCC、非L型VDCC以及L型VDCC的亚型，它们参与精子的生理过程，尤其是精子的运动。
