Martins Luciane, Leoni Suzana Garcia, Friguglietti Celso U M, Ward Laura Sterian, Kulcsar Marco Aurélio V, Kimura Edna Teruko
Departamento de Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade de São Paulo.
Arq Bras Endocrinol Metabol. 2006 Dec;50(6):1075-81. doi: 10.1590/s0004-27302006000600014.
Galectin-3 is a multifunctional protein highly expressed in thyroid cancer. The galectin-3 gene (LGALS3) has several annotated candidates SNPs, however the relationship between galectin-3 SNPs and specific phenotypic variations relevant to health has not been evaluated. In this study, we investigated SNPs in the galectin-3 gene and a putative association with thyroid tumorigenesis. The presence of LGALS3 SNPs in thyroid carcinoma cell lines (NPA, TPC-1, WRO, ARO), thyroid tissues of 55 patients with multinodular goiter or papillary carcinoma diagnosis and lymphocytes of peripheral blood of 45 healthy individuals was evaluated by sequencing and SSCP. The analysis of LGALS3 coding sequence showed that the T98P site presents a great genotypic variation, since we observed both homozygous (AA or CC) and heterozygous (AC) patterns. In thyroid carcinoma cell lines, the genotype of NPA in the LGALS3 T98P site is CC, while TPC-1, WRO and ARO are AC. The genotypic frequency of T98P SNP observed in multinodular goiter (AC= 67%; AA= 23%; CC= 10%) and papillary carcinoma (AC= 68%; AA= 20%; CC= 12%) were similar to the frequency observed in the control population (AC= 60%, AA= 24%, CC= 16%). In conclusion, no association between LGALS3 T98P genotype and the phenotype of the benign or malignant thyroid tumor was observed.
半乳糖凝集素-3是一种在甲状腺癌中高表达的多功能蛋白质。半乳糖凝集素-3基因（LGALS3）有多个注释的候选单核苷酸多态性（SNP），然而，半乳糖凝集素-3的SNP与健康相关的特定表型变异之间的关系尚未得到评估。在本研究中，我们调查了半乳糖凝集素-3基因中的SNP及其与甲状腺肿瘤发生的潜在关联。通过测序和单链构象多态性（SSCP）评估了甲状腺癌细胞系（NPA、TPC-1、WRO、ARO）、55例诊断为多结节性甲状腺肿或乳头状癌患者的甲状腺组织以及45名健康个体外周血淋巴细胞中LGALS3 SNP的存在情况。LGALS3编码序列分析表明，T98P位点存在很大的基因型变异，因为我们观察到了纯合子（AA或CC）和杂合子（AC）模式。在甲状腺癌细胞系中，LGALS3 T98P位点NPA的基因型为CC，而TPC-1、WRO和ARO为AC。在多结节性甲状腺肿（AC = 67%；AA = 23%；CC = 10%）和乳头状癌（AC = 68%；AA = 20%；CC = 12%）中观察到的T98P SNP基因型频率与在对照人群中观察到的频率（AC = 60%，AA = 24%，CC = 16%）相似。总之，未观察到LGALS3 T98P基因型与良性或恶性甲状腺肿瘤表型之间存在关联。
