Defoirdt Tom, Halet Dirk, Vervaeren Han, Boon Nico, Van de Wiele Tom, Sorgeloos Patrick, Bossier Peter, Verstraete Willy
Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, 9000 Gent, Belgium.
Environ Microbiol. 2007 Feb;9(2):445-52. doi: 10.1111/j.1462-2920.2006.01161.x.
Infections caused by antibiotic-resistant luminescent Vibrios can cause dramatic losses in aquaculture. In this study, the short-chain fatty acid beta-hydroxybutyrate and its polymer poly-beta-hydroxybutyrate were investigated as possible new biocontrol agents. beta-Hydroxybutyrate was shown to completely inhibit the growth of pathogenic Vibrio campbelli at 100 mM. Moreover, the addition of 100 mM of this fatty acid to the culture water of Artemia nauplii infected with the V. campbelli strain significantly increased the survival of the nauplii. As Artemia is a non-selective and particle filter feeder, we also investigated whether poly-beta-hydroxybutyrate particles could be used to protect Artemia from the pathogenic V. campbellii. The addition of 100 mg l(-1) poly-beta-hydroxybutyrate or more to the Artemia culture water offered a preventive and curative protection from the pathogen as a significantly enhanced survival was noticed. If added as a preventive treatment, a complete protection of infected nauplii (no significant mortality compared with uninfected nauplii) was observed at 1000 mg l(-1) poly-beta-hydroxybutyrate. Our data indicate that the use of poly-beta-hydroxybutyrate might constitute an ecologically and economically sustainable alternative strategy to fight infections in aquaculture.
由耐抗生素发光弧菌引起的感染会给水产养殖造成巨大损失。在本研究中，对短链脂肪酸β-羟基丁酸及其聚合物聚-β-羟基丁酸作为可能的新型生物防治剂进行了研究。结果表明，100 mM的β-羟基丁酸能完全抑制致病性坎氏弧菌的生长。此外，向感染坎氏弧菌菌株的卤虫无节幼体养殖水中添加100 mM这种脂肪酸，可显著提高无节幼体的存活率。由于卤虫是一种非选择性的颗粒滤食性动物，我们还研究了聚-β-羟基丁酸颗粒是否可用于保护卤虫免受致病性坎氏弧菌的侵害。向卤虫养殖水中添加100 mg l(-1)或更多的聚-β-羟基丁酸，可对病原体提供预防和治疗保护，因为观察到存活率显著提高。如果作为预防性处理添加，在1000 mg l(-1)聚-β-羟基丁酸时，可观察到对感染无节幼体的完全保护（与未感染无节幼体相比无显著死亡率）。我们的数据表明，使用聚-β-羟基丁酸可能构成一种在生态和经济上可持续的替代策略，以对抗水产养殖中的感染。
