Varesio L, Cappuccinelli P, Forni G
Cancer Res. 1975 Dec;35(12):3558-65.
The activity of 7S immunoglobulins (Ig) antibody to RNA, obtained in rabbits after a prolonged immunization with RNA-methylated bovine serum albumin complex was evaluated in vitro on normal (3T3) and simian virus 40-transformed (SV 3T3) mouse fibroblasts. The presence of anti-RNA antibody in the culture medium inhibited both the SV 3T3 cell proliferation and the [3H]thymidine incorporation. In contrast, an increased [3H]uridine incorporation was evident within 48 and 96 hr of culture. No significant modification in these 3 parameters was observed in 3T3 cultures treated in the same manner. Both 3T3 and SV 3T3 showed cytoplasmic fluorescence when cultured in the presence of fluoresceinated anti-RNA Ig. However, with the indirect fluorescence technique anti-RNA Ig were detected in SV 3T3 cytoplasm only. These data suggest that anti-RNA Ig were taken up by both 3T3 and SV 3T3, but only in SV 3T3 did the anti-RNA Ig retain their antigenic properties and block cellular proliferation.
用甲基化RNA - 牛血清白蛋白复合物对家兔进行长时间免疫后获得的7S免疫球蛋白（Ig）抗RNA抗体活性，在体外对正常（3T3）和猿猴病毒40转化（SV 3T3）的小鼠成纤维细胞进行了评估。培养基中抗RNA抗体的存在抑制了SV 3T3细胞的增殖以及[3H]胸苷掺入。相反，在培养48小时和96小时内，[3H]尿苷掺入明显增加。以相同方式处理的3T3培养物中，这三个参数未观察到明显变化。当在荧光素标记的抗RNA Ig存在下培养时，3T3和SV 3T3均显示出细胞质荧光。然而，采用间接荧光技术时，仅在SV 3T3细胞质中检测到抗RNA Ig。这些数据表明，抗RNA Ig被3T3和SV 3T3摄取，但仅在SV 3T3中抗RNA Ig保留其抗原特性并阻断细胞增殖。
