Garulli Bruno, Meola Monica, Stillitano Maria Giuseppina, Kawaoka Yoshihiro, Castrucci Maria Rita
Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
Virology. 2007 May 10;361(2):274-82. doi: 10.1016/j.virol.2006.12.001. Epub 2007 Jan 11.
Effective vaccination strategies for infectious diseases take into account the induction, long-term maintenance and recall of memory T-cell populations. To understand the immunological cross-talk within the mucosal compartments, we compared intranasal to vaginal immunization and demonstrated that vaginal infection of BALB/c mice with influenza A virus provides protective mucosal immunity against both homosubtypic and heterosubtypic virus challenge in the respiratory tract. We found that, prior to the viral challenge, in vaginally primed mice, antigen-specific CD8+ T cells were not detected in the lung airways and levels of serum antibodies were lower than those observed in intranasally immunized mice. However, following pulmonary challenge, NP147-specific CD8+ T cells were recruited and amplified in vaginally primed mice to the same extent as those in intranasally primed mice. Thus, the long-term memory immune response elicited by vaginal immunization with influenza virus is efficiently recalled and offers reasonable protection against infection in the respiratory tract.
针对传染病的有效疫苗接种策略会考虑记忆性T细胞群体的诱导、长期维持和再次激活。为了解黏膜区室中的免疫相互作用，我们比较了鼻内免疫和阴道免疫，并证明用甲型流感病毒对BALB/c小鼠进行阴道感染可提供针对呼吸道同型和异型病毒攻击的保护性黏膜免疫。我们发现，在病毒攻击前，在经阴道启动的小鼠中，肺气道中未检测到抗原特异性CD8+ T细胞，血清抗体水平低于鼻内免疫小鼠中观察到的水平。然而，在肺部攻击后，NP147特异性CD8+ T细胞在经阴道启动的小鼠中被募集并扩增到与经鼻内启动的小鼠相同的程度。因此，流感病毒阴道免疫引发的长期记忆免疫反应能有效再次激活，并为呼吸道感染提供合理的保护。
