Ebina T, Ishida N
Cancer Res. 1975 Dec;35(12):3705-9.
The effect of an antitumor antibiotic, neocarzinostatin (NCS), on the formation of microtubular paracrystals (PC) induced by vinblastine sulfate, 10 mug/ml, in HeLa-S3 cells was examined by phase-contrast microscopy. The pretreatment of HeLa-S3 cells with NCS, 5 to 50 mug/ml, for 4 hr prevented the PC formation, and there was a dose response of NCS to the degree of inhibition. When the same inhibitory effect on PC formation was examined with other antitumor agents at high doses (50 mug/ml), colchicine was found to be one of the most effective agents, like NCS. Puromycin, antimycin, adriamycin, cytochalasin B, and cycloheximide revealed moderate activity, and the other antibiotics, such as mitomycin C, bleomycin, and rifampicin, did not show any effect at all. NCS was a unique antibiotic that inhibited PC formation among inhibitors of DNA synthesis. It was suggested that NCS affects the microfibrillar-microtubular proteins system in vivo, resulting in the inhibitions of organization of spindle fibers from microtubules at the G2 phase in HeLa cells.
通过相差显微镜检查了抗肿瘤抗生素新制癌菌素（NCS）对硫酸长春碱（10微克/毫升）诱导HeLa-S3细胞形成微管副晶体（PC）的影响。用5至50微克/毫升的NCS预处理HeLa-S3细胞4小时可防止PC形成，并且NCS对抑制程度存在剂量反应。当用其他高剂量（50微克/毫升）的抗肿瘤药物检查对PC形成的相同抑制作用时，发现秋水仙碱与NCS一样是最有效的药物之一。嘌呤霉素、抗霉素、阿霉素、细胞松弛素B和环己酰亚胺显示出中等活性，而其他抗生素，如丝裂霉素C、博来霉素和利福平则完全没有效果。在DNA合成抑制剂中，NCS是一种独特的抑制PC形成的抗生素。提示NCS在体内影响微纤丝-微管蛋白系统，导致HeLa细胞在G2期微管纺锤体纤维的组装受到抑制。
