Delcasso Sebastien, Jeantet Yannick, Cho Yoon H
Laboratory of Cognitive Neuroscience, CNRS UMR 5106, Avenue des Facultés 33405 Talence Cedex, France.
Behav Brain Res. 2007 Mar 28;178(2):200-7. doi: 10.1016/j.bbr.2006.12.019. Epub 2006 Dec 21.
As part of ongoing efforts to develop fully automated and standardized behavioral tasks to probe cognitive and mnemonic capabilities of mice, we have constructed a new rectangular operant chamber. The chamber contains numerous nose poke holes, distributed over three of its inner walls that are identifiable by their spatial locations. Using this apparatus, we have developed a 'spatial' memory task using a successive reversal discrimination paradigm. Mice learn to discriminate, by trial and error, the position of a single valid hole during a Presentation session wherein they obtained a maximum of 20 reinforcements or 15 min time elapsed. Following a delay interval, they were resubmitted to the same task (Test) using the same reinforced hole. Results indicated that C57BL/6 mice exhibited a significant improvement during the Test, the magnitude of the improvement (memory savings) being dependent on the length of retention intervals ranging from 5 min to 24h. In addition, discrimination performance was sensitive to scopolamine in a dose dependent manner. The simplicity in task set up and the minimal labor and space requirements make this task suitable for high throughput behavioral characterization of genetically modified mice.
作为持续努力开发用于探究小鼠认知和记忆能力的全自动标准化行为任务的一部分，我们构建了一个新的矩形操作箱。该操作箱包含许多鼻触孔，分布在其三个内壁上，可通过其空间位置识别。使用该装置，我们利用连续反转辨别范式开发了一项“空间”记忆任务。在呈现阶段，小鼠通过反复试验学会辨别单个有效孔的位置，在此期间它们最多可获得20次强化或经过15分钟。经过延迟间隔后，它们使用相同的强化孔再次接受相同任务（测试）。结果表明，C57BL/6小鼠在测试期间表现出显著改善，改善程度（记忆节省）取决于5分钟至24小时的保留间隔长度。此外，辨别性能对东莨菪碱呈剂量依赖性敏感。任务设置简单，劳动力和空间需求最小，使得该任务适用于转基因小鼠的高通量行为表征。
