Chung D W, Zhang J A, Tan C K, Davie E W, So A G, Downey K M
Department of Biochemistry, University of Washington, Seattle 98195.
Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11197-201. doi: 10.1073/pnas.88.24.11197.
The catalytic subunit (Mr approximately 124,000) of human DNA polymerase delta has been cloned by PCR using poly(A)+ RNA from HepG2 cells and primers designed from the amino acid sequence of regions highly conserved between bovine and yeast DNA polymerase delta. The human cDNA was 3443 nucleotides in length and coded for a polypeptide of 1107 amino acids. The enzyme was 94% identical to bovine DNA polymerase delta and contained the numerous highly conserved regions previously observed in the bovine and yeast enzymes. The human enzyme also contained two putative zinc-finger domains in the carboxyl end of the molecule, as well as a putative nuclear localization signal at the amino-terminal end. The gene coding for human DNA polymerase delta was localized to chromosome 19.
利用从HepG2细胞中提取的聚腺苷酸加尾RNA（poly(A)+ RNA），通过聚合酶链式反应（PCR），并使用根据牛和酵母DNA聚合酶δ之间高度保守区域的氨基酸序列设计的引物，克隆出了人类DNA聚合酶δ的催化亚基（分子量约为124,000）。人类cDNA长度为3443个核苷酸，编码一个由1107个氨基酸组成的多肽。该酶与牛DNA聚合酶δ有94%的同源性，并且含有先前在牛和酵母酶中观察到的众多高度保守区域。人类酶在分子的羧基末端还含有两个假定的锌指结构域，以及在氨基末端的一个假定的核定位信号。编码人类DNA聚合酶δ的基因定位于19号染色体。
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