Ichikawa Y, Shimizu H, Yoshida M, Takaya M, Arimori S
Department of Internal Medicine, Tokai University School of Medicine, Kanagawa, Japan.
Clin Exp Rheumatol. 1991 Nov-Dec;9(6):603-9.
T cells bearing gamma/delta T cell receptor (gamma/delta + T cells) and their expression of activation antigen (HLA-DR) or the marker of natural killer (NK) cells (CD56), were examined in the peripheral blood lymphocytes (PBL) from twenty-two patients with Sjögren's syndrome (SS) by three-color flowcytometry to elucidate possible pathological roles of the T cell subset in SS. The frequency of gamma/delta + T cells in PBL was not elevated in SS patients, while that of gamma/delta - T cells, which are T cells bearing the alpha/beta T cell receptor (alpha/beta + T cells), was significantly low in the patients, as compared with 22 healthy controls. We found that the proportions of activated cells (HLA-DR+) in both the gamma/delta + and alpha/beta+T cell subsets were significantly higher in the patients than in the controls. The proportions of HLA-DR+ cells in cells in both patients and controls. Furthermore, the frequency of activated cells in both T cell subsets correlated with the duration of disease in SS patients. However, no difference was found in the percentages of total CD56+ cells, CD56+CD3- cells (true NK cells), CD56+CD3+T cells, CD56+gamma/delta+T cells, or CD56-gamma/delta+T cells between the patients and controls. The above results indicate that immunologic activation in SS patients is progressive and involves both alpha/beta+ and gamma/delta+ T cell subsets.
采用三色流式细胞术检测了22例干燥综合征（SS）患者外周血淋巴细胞（PBL）中携带γ/δ T细胞受体的T细胞（γ/δ + T细胞）及其活化抗原（HLA-DR）或自然杀伤（NK）细胞标志物（CD56）的表达，以阐明该T细胞亚群在SS中可能的病理作用。SS患者PBL中γ/δ + T细胞的频率未升高，而携带α/β T细胞受体的T细胞（α/β + T细胞）即γ/δ - T细胞的频率在患者中显著低于22名健康对照者。我们发现，患者γ/δ +和α/β + T细胞亚群中活化细胞（HLA-DR+）的比例均显著高于对照者。患者和对照者中HLA-DR+细胞的比例。此外，两个T细胞亚群中活化细胞的频率与SS患者的病程相关。然而，患者与对照者之间总CD56+细胞、CD56+CD3-细胞（真正的NK细胞）、CD56+CD3+T细胞、CD56+γ/δ + T细胞或CD56-γ/δ + T细胞的百分比均无差异。上述结果表明，SS患者的免疫激活是进行性的，涉及α/β +和γ/δ + T细胞亚群。
