Hermans Nina, Cos Paul, De Meyer Guido R Y, Maes Louis, Pieters Luc, Vanden Berghe Dirk, Vlietinck Arnold J, De Bruyne Tess
Laboratory of Nutrition and Functional Food Science, Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium.
J Pharm Pharmacol. 2007 Jan;59(1):131-6. doi: 10.1211/jpp.59.1.0017.
Although many compounds have already been tested in-vitro to determine their antioxidant profile, it is necessary to investigate the in-vivo effect of potential antioxidants. However, representative models of systemic oxidative stress have been poorly studied. Here, different potential systemic oxidative stress animal models have been investigated. These included a vitamin E-deficient rat, a diabetic rat and an atherosclerotic rabbit model. Plasma/serum malondialdehyde was measured as a parameter of oxidative damage. Plasma/serum fat-soluble antioxidants were determined as markers of antioxidant defence. We demonstrated that vitamin E-deficient rats were not suitable as a model of systemic oxidative stress, whereas diabetic and atherosclerotic animals showed increased systemic oxidative damage, as reflected by significantly augmented plasma/serum malondialdehyde. Moreover, plasma coenzyme Q9 increased by 80% in diabetic rats, confirming systemic oxidative stress. In view of these observations and economically favouring factors, the diabetic rat appeared to be the most appropriate systemic oxidative stress model. These findings have provided important information concerning systemic oxidative stress animal models for the in-vivo study of antioxidants.
尽管许多化合物已经在体外进行了测试以确定其抗氧化特性，但有必要研究潜在抗氧化剂的体内作用。然而，系统性氧化应激的代表性模型研究较少。在此，对不同的潜在系统性氧化应激动物模型进行了研究。这些模型包括维生素E缺乏大鼠、糖尿病大鼠和动脉粥样硬化兔模型。测定血浆/血清丙二醛作为氧化损伤的参数。测定血浆/血清脂溶性抗氧化剂作为抗氧化防御的标志物。我们证明，维生素E缺乏大鼠不适合作为系统性氧化应激模型，而糖尿病和动脉粥样硬化动物表现出系统性氧化损伤增加，这通过血浆/血清丙二醛显著增加得以体现。此外，糖尿病大鼠血浆辅酶Q9增加了80%，证实了系统性氧化应激。鉴于这些观察结果和经济因素，糖尿病大鼠似乎是最合适的系统性氧化应激模型。这些发现为抗氧化剂体内研究的系统性氧化应激动物模型提供了重要信息。
