Murakami M, Umeda M, Kudo I, Inoue K
Department of Health Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Int Arch Allergy Appl Immunol. 1991;96(2):156-60. doi: 10.1159/000235487.
Lysophosphatidylserine (lysoPS) is known to enhance IgE-mediated activation of rodent connective tissue mast cells (CTMCs). In the present study, we investigated the effect of lysoPS on degranulation of interleukin-3-dependent mouse bone marrow-derived mucosal mast cells (BMMCs) and of their CTMC-like differentiated cells. In the absence of lysoPS, BMMCs released approximately 20% of their histamine when sensitized with anti-dinitrophenyl (DNP) IgE and challenged with DNP-conjugated antigen. When stimulated in the presence of lysoPS, no appreciable enhancement was observed. On the other hand, histamine release from BMMCs, which had differentiated to CTMC-like cells by co-culture with 3T3 fibroblasts, was enhanced 2- to 3-fold by the addition of lysoPS. The maximum potentiation was observed at 5 x 10(-6) M lysoPS. These results suggest that mast cells might acquire their dependence on exogenous lysoPS during differentiation from mucosal mast cells to CTMC-like cells.
溶血磷脂酰丝氨酸（lysoPS）已知可增强啮齿动物结缔组织肥大细胞（CTMC）的IgE介导的活化。在本研究中，我们研究了lysoPS对白细胞介素-3依赖性小鼠骨髓来源的黏膜肥大细胞（BMMC）及其CTMC样分化细胞脱颗粒的影响。在没有lysoPS的情况下，当用抗二硝基苯基（DNP）IgE致敏并用DNP偶联抗原攻击时，BMMC释放其约20%的组胺。当在lysoPS存在下刺激时，未观察到明显增强。另一方面，通过与3T3成纤维细胞共培养分化为CTMC样细胞的BMMC的组胺释放，通过添加lysoPS增强了2至3倍。在5×10^(-6) M lysoPS时观察到最大增强作用。这些结果表明，肥大细胞在从黏膜肥大细胞向CTMC样细胞分化过程中可能获得对外源lysoPS的依赖性。
