II组磷脂酶A2抑制剂可抑制大鼠腹膜肥大细胞的溶血磷脂酰丝氨酸依赖性脱颗粒。

Group II phospholipase A2 inhibitors suppressed lysophosphatidylserine-dependent degranulation of rat peritoneal mast cells.

作者信息

Murakami M, Kudo I, Fujimori Y, Suga H, Inoue K

机构信息

Department of Health Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.

出版信息

Biochem Biophys Res Commun. 1991 Dec 16;181(2):714-21. doi: 10.1016/0006-291x(91)91249-c.

DOI:10.1016/0006-291x(91)91249-c

PMID:1721808

Abstract

Rat peritoneal mast cells were sensitized with IgE and challenged with the specific antigen in the presence of lysophosphatidylserine (lysoPS), an essential co-factor for rodent connective tissue mast cell degranulation, and the effects of phospholipase A2 inhibitors were examined. Mepacrine, a known inhibitor of phospholipase A2, at concentrations below 10(-5) M and anti-rat 14-kDa group II phospholipase A2 antibody inhibited histamine release, while they did not affect the prostaglandin generation. Like histamine release, prostaglandin generation in IgE- and antigen- challenged rat peritoneal mast cells was dependent on the presence of lysoPS. These results indicate that 14-kDa group II phospholipase A2 may play an essential role in IgE-, antigen-, and lysoPS-dependent degranulation process of rat peritoneal mast cells and that the mechanism whereby it participates may not be due to the production of lysoPS from PS in mast cell membranes.

摘要

用IgE使大鼠腹膜肥大细胞致敏，并在溶血磷脂酰丝氨酸（lysoPS，啮齿动物结缔组织肥大细胞脱颗粒的必需辅助因子）存在的情况下用特异性抗原进行激发，然后检测磷脂酶A2抑制剂的作用。已知的磷脂酶A2抑制剂米帕林，在浓度低于10^(-5) M时以及抗大鼠14-kDa II组磷脂酶A2抗体可抑制组胺释放，而它们不影响前列腺素的生成。与组胺释放一样，IgE和抗原激发的大鼠腹膜肥大细胞中前列腺素的生成依赖于lysoPS的存在。这些结果表明，14-kDa II组磷脂酶A2可能在大鼠腹膜肥大细胞的IgE、抗原和lysoPS依赖性脱颗粒过程中起重要作用，并且其参与的机制可能不是由于肥大细胞膜中PS产生lysoPS。