[结核分枝杆菌cfpl0-esat6融合基因重组整合穿梭质粒的构建及其在卡介苗中的表达]

[Construction of the recombinant integrating shuttle plasmid with cfpl0-esat6 fusion gene of Mycobacterium tuberculosis and its expression in BCG].

作者信息

Wang Xiaoying, Bao Lang, Zhao Mingcai, Zhang Huidong, Long Yang

机构信息

Research Unit of Infection and Immunity, West China Center of Medical Sciences, Sichuan University, Chengdu 610041, China.

出版信息

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2006 Dec;23(6):1298-302.

PMID:17228730

Abstract

This study was conducted to amplify the cfpl0-esat6 fusion gene by SOE and insert into the integrating shuttle plasmid pMV361 to form the recombinant plasmid. Then another recombinant plasmid was constructed by insertinga-A g signal sequence of BCG. The two recombinant plasmids were introduced into BCG and the induced products from recombinant BCG were analyzed. In conclusion,the successful construction of rBCG expressing the fusion protein CFP10-ESAT6 will be the base of the development of novel Mycobacterium tuberclosis vaccines.

摘要

本研究旨在通过重叠延伸PCR（SOE）扩增cfp10-esat6融合基因，并将其插入整合穿梭质粒pMV361中以形成重组质粒。然后通过插入卡介苗（BCG）的α-Ag信号序列构建另一种重组质粒。将这两种重组质粒导入卡介苗，并对重组卡介苗的诱导产物进行分析。总之，成功构建表达融合蛋白CFP10-ESAT6的重组卡介苗将为新型结核病疫苗的开发奠定基础。