共表达Ag85B、ESAT-6和小鼠干扰素-γ的重组卡介苗对C57BL/6小鼠的结核分枝杆菌感染具有有效的保护作用。
Recombinant BCG coexpressing Ag85B, ESAT-6 and mouse-IFN-gamma confers effective protection against Mycobacterium tuberculosis in C57BL/6 mice.
作者信息
Xu Ying, Zhu Bingdong, Wang Qingzhong, Chen Jiazhen, Qie Yaqing, Wang Jiuling, Wang Hongyan, Wang Baolin, Wang Honghai
机构信息
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, China.
出版信息
FEMS Immunol Med Microbiol. 2007 Dec;51(3):480-7. doi: 10.1111/j.1574-695X.2007.00322.x. Epub 2007 Oct 4.
In this study, the protective efficacy of a novel recombinant bacille Calmette Géurin (BCG) strain (rBCG-AEI) expressing fusion protein the antigen 85B (Ag85B)- the 6-kDa early secreted antigen target (ESAT-6)-IFN-gamma against Mycobacterium tuberculosis H37Rv in mice was evaluated. The immunogenicity study showed that rBCG-AEI could induce higher specific antibody titers and significantly increase cellular immune response than BCG, or rBCG-A strain (expressing Ag85B), or rBCG-AE strain (expressing fusion protein Ag85B-ESAT-6). The protective experiment demonstrated that rBCG-AEI could confer similar or even better protective efficacy against M. tuberculosis infection compared with others in organ bacterial loads, lung histopathology and net weight gain or loss. The results suggested that rBCG-AEI is a potential candidate for further study.
在本研究中，评估了一种表达融合蛋白抗原85B（Ag85B）-6 kDa早期分泌抗原靶标（ESAT-6）-干扰素-γ的新型重组卡介苗（rBCG）菌株（rBCG-AEI）对小鼠结核分枝杆菌H37Rv的保护效力。免疫原性研究表明，与卡介苗、rBCG-A菌株（表达Ag85B）或rBCG-AE菌株（表达融合蛋白Ag85B-ESAT-6）相比，rBCG-AEI可诱导更高的特异性抗体滴度，并显著增强细胞免疫反应。保护性实验表明，在器官细菌载量、肺组织病理学以及净体重增加或减少方面，与其他菌株相比，rBCG-AEI对结核分枝杆菌感染可赋予相似甚至更好的保护效力。结果表明，rBCG-AEI是进一步研究的潜在候选菌株。
Zotero 插件