单核吞噬细胞中HIV的差异复制及HIV诱导的干扰素产生：与细胞成熟的关系

Differential HIV replication and HIV-induced interferon production in mononuclear phagocytes: relationship to cell maturation.

作者信息

Mosborg-Petersen P, Toth F D, Zachar V, Villadsen J A, Nørskov-Lauritsen N, Aboagye-Mathiesen G, Chermann J C, Ebbesen P

机构信息

Department of Virus and Cancer, Danish Cancer Society, Aarhus, Denmark.

出版信息

Res Virol. 1991 Sep-Oct;142(5):353-61. doi: 10.1016/0923-2516(91)90002-k.

DOI:10.1016/0923-2516(91)90002-k

PMID:1722899

Abstract

We have investigated the replication of human immunodeficiency virus (HIV) and HIV-induced interferon (IFN) production in human mononuclear phagocytes at 2 different stages of in vitro maturation. Blood monocytes and monocyte-derived macrophages from 6 healthy, HIV-seronegative donors were challenged with HIV1IIIB and HIV2ROD. Freshly separated monocytes produced IFN when inoculated with both HIV types. In these cultures, an inverse correlation was observed between the amount of IFN production and the rate of HIV replication. In contrast to the monocytes, 5-day-old monocyte-derived macrophages did not produce IFN when challenged with HIV, but a significant replication of HIV1IIIB and HIV2ROD was found in all cultures.

摘要

我们研究了人免疫缺陷病毒（HIV）在体外成熟的两个不同阶段在人单核吞噬细胞中的复制情况以及HIV诱导的干扰素（IFN）产生情况。来自6名健康的、HIV血清阴性供体的血液单核细胞和单核细胞衍生的巨噬细胞受到HIV1IIIB和HIV2ROD的攻击。新鲜分离的单核细胞在接种这两种HIV类型时都会产生IFN。在这些培养物中，观察到IFN产生量与HIV复制率之间呈负相关。与单核细胞不同，5日龄单核细胞衍生的巨噬细胞在受到HIV攻击时不产生IFN，但在所有培养物中都发现了HIV1IIIB和HIV2ROD的显著复制。