通过单链构象多态性分析对鱼类病原菌柱状黄杆菌分离株进行分子分型。

Molecular typing of isolates of the fish pathogen, Flavobacterium columnare, by single-strand conformation polymorphism analysis.

作者信息

Olivares-Fuster Oscar, Shoemaker Craig A, Klesius Phillip H, Arias Covadonga R

机构信息

Department of Fisheries and Allied Aquacultures, Auburn University, Auburn, AL 36849, USA.

出版信息

FEMS Microbiol Lett. 2007 Apr;269(1):63-9. doi: 10.1111/j.1574-6968.2006.00605.x. Epub 2007 Jan 11.

DOI:10.1111/j.1574-6968.2006.00605.x

PMID:17229061

Abstract

Flavobacterium columnare intraspecies diversity was revealed by analyzing the 16S rRNA gene and the 16S-23S internal spacer region (ISR). Standard restriction fragment length polymorphism (RFLP) of these sequences was compared with single-strand conformation polymorphism (SSCP). Diversity indexes showed that both 16S-SSCP and ISR-SSCP improved resolution (D>or=0.9) when compared with standard RFLP. ISR-SSCP offered a simpler banding pattern than 16S-SSCP while providing high discrimination between isolates. SSCP analysis of rRNA genes proved to be a simple, rapid, and cost-effective method for routine fingerprinting of F. columnare.

摘要

通过分析16S rRNA基因和16S - 23S内部间隔区（ISR）揭示了柱状黄杆菌种内多样性。将这些序列的标准限制性片段长度多态性（RFLP）与单链构象多态性（SSCP）进行比较。多样性指数表明，与标准RFLP相比，16S - SSCP和ISR - SSCP均提高了分辨率（D≥0.9）。ISR - SSCP提供了比16S - SSCP更简单的条带模式，同时在分离株之间具有高鉴别力。rRNA基因的SSCP分析被证明是一种用于柱状黄杆菌常规指纹图谱分析的简单、快速且经济高效的方法。