Ohta Fumio, Takagi Tomo, Sato Hiroyuki, Ignarro Louis J
Laboratory of Amino Acid Application, AminoScience Laboratories, Ajinomoto Co., Inc., 1-1 Suzuki-cho Kawasaki-ku, Kawasaki 210-8681, Japan.
Proc Natl Acad Sci U S A. 2007 Jan 23;104(4):1407-11. doi: 10.1073/pnas.0610207104. Epub 2007 Jan 17.
The objective of this work was to evaluate the influence of exogenous L-arginine on the capillary blood flow of peripheral tissues of normotensive subjects. Rats were anesthetized with sodium pentobarbital, and the blood flow of femoral, dorsal, and ventral skin and gastrocnemius and soleus muscle was measured by laser Doppler flow and microsphere methods to compare the blood flow before and after the L-arginine infusion. L-arginine lowered the mean blood pressure in a dose-dependent manner, but a statistically significant reduction in mean blood pressure was detected only at a high dose of 500 mg/kg of body weight. The significant blood flow increment was detected after the L-arginine infusion at doses of 50 and 150 mg/kg without causing hypotension. Nicardipine, a calcium channel blocker, also increased the skin blood flow, but the blood flow increment and blood pressure fall were comparable. A significant increment in microperfusion was detected in gastrocnemius, soleus muscle, and ventral skin compared with control group by the microsphere method. No adverse effects were observed during L-arginine and microsphere infusion. The present work indicates that l-arginine infusion increases muscle capillary blood flow in rats that are not performing exercise. Supplementation with l-arginine might provide additional blood flow at rest and during exercise and result in the improvement of muscle performance and exercise capacity.
这项工作的目的是评估外源性L-精氨酸对血压正常受试者外周组织毛细血管血流的影响。用戊巴比妥钠麻醉大鼠，通过激光多普勒血流仪和微球法测量股部、背部和腹部皮肤以及腓肠肌和比目鱼肌的血流，以比较L-精氨酸输注前后的血流情况。L-精氨酸以剂量依赖性方式降低平均血压，但仅在500mg/kg体重的高剂量下才检测到平均血压有统计学意义的降低。在输注50mg/kg和150mg/kg剂量的L-精氨酸后检测到显著的血流增加，且未引起低血压。钙通道阻滞剂尼卡地平也增加了皮肤血流，但血流增加和血压下降情况相当。通过微球法检测发现，与对照组相比，腓肠肌、比目鱼肌和腹部皮肤的微灌注有显著增加。在输注L-精氨酸和微球过程中未观察到不良反应。目前的研究表明，输注L-精氨酸可增加未进行运动的大鼠的肌肉毛细血管血流。补充L-精氨酸可能在休息和运动期间提供额外的血流，并导致肌肉性能和运动能力的改善。
