Michel P, Van Velthoven R, Petein M, Gras S, Etievant C, Pasteels J L, Kiss R
Department of Surgery, Hospital St Pierre, Brussels, Belgium.
Anticancer Res. 1991 Nov-Dec;11(6):2075-8.
We studied the suramin-induced influence on the cell proliferation of 16 benign and 6 malignant lesions of the human prostate maintained in vitro as organ cultures. The cell proliferation was assessed by nuclear labeling with tritiated thymidine autoradiography. We also studied the dihydrotestosterone (DHT)-and platelet-derived growth factor (PDGF)-induced modulation of suramin influence on such prostate organ culture cell proliferation. Our results indicate that more than half of the benign prostatic tissues showed cell proliferation which was modulated by DHT and/or PDGF, while none of the six carcinomas responded to such hormonal stimulation. Suramin alone inhibited the cell proliferation of only 19% of the prostate organ culture under study, while in combination with DHT and/or PDGF this inhibition level reached 48%. However, we occasionally observed that S alone or in combination with DHT and/or PDGF was also able to stimulate prostate cell proliferation. We think that organ cultures of human prostatic tissues might represent a helpful pre-clinical tool to study the anti-tumoral influence of suramin, which is a new antineoplastic generative compound.
我们研究了苏拉明对体外作为器官培养物保存的16例人类前列腺良性病变和6例恶性病变细胞增殖的影响。通过用氚标记的胸腺嘧啶核苷放射自显影术进行核标记来评估细胞增殖。我们还研究了二氢睾酮（DHT）和血小板衍生生长因子（PDGF）对苏拉明影响此类前列腺器官培养细胞增殖的调节作用。我们的结果表明，超过一半的良性前列腺组织显示出细胞增殖，其受到DHT和/或PDGF的调节，而6例癌组织均未对这种激素刺激产生反应。单独使用苏拉明仅抑制了所研究的19%的前列腺器官培养物的细胞增殖，而与DHT和/或PDGF联合使用时，这种抑制水平达到48%。然而，我们偶尔观察到，单独使用S或与DHT和/或PDGF联合使用时，也能够刺激前列腺细胞增殖。我们认为，人类前列腺组织的器官培养可能是一种有用的临床前工具，用于研究苏拉明的抗肿瘤影响，苏拉明是一种新型抗肿瘤生成化合物。
