Okamoto T, Kanamaru A, Kakishita E, Nagai K
2nd Department of Internal Medicine, Hyogo College of Medicine, Japan.
Int J Hematol. 1991 Aug;54(4):299-306.
We cultured bone marrow cells from patients receiving bone marrow transplantation (BMT) to assay bone marrow fibroblast colony-forming cells (CFU-F) and hematopoietic progenitors (CFU-mix, CFU-C, BFU-E and CFU-E), and compared the mean values obtained from patients with and without graft-versus-host disease (GVHD). The value of CFU-F colonies from 15 patients was always less in patients with acute GVHD Grade II,III than in those with Grade 0,I over the period 30 to 110 days after BMT. The CFU-F colonies from patients with Grade 0,I consisted of approximately the same number of small and large colonies, whereas virtually all CFU-F colonies from patients with Grade II,III were small. Fibroblasts collected from bone marrow cells cultured for 2-3 weeks were incubated with IL-1 (50 U/ml) for 24 h. The concentrations of G-CSF in the culture supernatants from patients with Grade II,III were higher than in those with Grade 0,I. The results of assays of hematopoietic progenitors from 48 patients showed that the number of hematopoietic progenitors decreased as the severity of acute GVHD increased. These results suggest that the myelosuppression seen in GVHD may be associated with reduced numbers of CFU-F in bone marrow.
我们培养了接受骨髓移植（BMT）患者的骨髓细胞，以检测骨髓成纤维细胞集落形成细胞（CFU-F）和造血祖细胞（CFU-mix、CFU-C、BFU-E和CFU-E），并比较了有和没有移植物抗宿主病（GVHD）患者的检测均值。在BMT后30至110天期间，15例急性GVHD II、III级患者的CFU-F集落值始终低于0、I级患者。0、I级患者的CFU-F集落由数量大致相同的小集落和大集落组成，而II、III级患者的几乎所有CFU-F集落都是小集落。将从培养2-3周的骨髓细胞中收集的成纤维细胞与IL-1（50 U/ml）孵育24小时。II、III级患者培养上清液中G-CSF的浓度高于0、I级患者。对48例患者造血祖细胞的检测结果表明，随着急性GVHD严重程度的增加，造血祖细胞数量减少。这些结果表明，GVHD中出现的骨髓抑制可能与骨髓中CFU-F数量减少有关。
