三氯乙烯还原脱卤酶基因（tceA）的环境分布表明脱卤球菌属之间存在侧向基因转移。
Environmental distribution of the trichloroethene reductive dehalogenase gene (tceA) suggests lateral gene transfer among Dehalococcoides.
作者信息
Krajmalnik-Brown Rosa, Sung Youlboong, Ritalahti Kirsti M, Saunders F Michael, Löffler Frank E
机构信息
School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332-0512, USA.
出版信息
FEMS Microbiol Ecol. 2007 Jan;59(1):206-14. doi: 10.1111/j.1574-6941.2006.00243.x.
The trichloroethene reductive dehalogenase gene (tceA) of Dehalococcoides spp. was detected in 12 of 21 trichloroethene-to-ethene dechlorinating enrichment cultures established from aquifer and sediment samples collected from diverse geographic locations in the USA. Analysis of the tceA chromosomal regions indicated that the tceA genes shared greater than 95% sequence identity, and all shared identical tceAB spacer sequences and tceB genes downstream of tceA. A putative transposable element (PTE) was present 1077 bp downstream of the tceB stop codon in three of eight chromosomal regions analyzed. Sequence identity was interrupted downstream of tceB and upstream or downstream of the PTE, suggesting that intrachromosomal or interchromosomal transfer of tceAB had occurred.
在美国不同地理位置采集的含水层和沉积物样本所建立的21种三氯乙烯到乙烯脱氯富集培养物中，有12种检测到了脱卤球菌属的三氯乙烯还原脱卤酶基因（tceA）。对tceA染色体区域的分析表明，tceA基因的序列同一性大于95%，并且所有基因都共享相同的tceAB间隔序列以及tceA下游的tceB基因。在所分析的八个染色体区域中的三个区域，一个推定的转座元件（PTE）位于tceB终止密码子下游1077 bp处。tceB下游以及PTE的上游或下游的序列同一性被打断，这表明tceAB发生了染色体内或染色体间转移。
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