Ahmadzadeh Hossein, Andreyev Dmitry, Arriaga Edgar A, Thompson LaDora V
Department of Chemistry, California State Polytechnic University, Pomona, CA, USA.
J Gerontol A Biol Sci Med Sci. 2006 Dec;61(12):1211-8. doi: 10.1093/gerona/61.12.1211.
Capillary electrophoresis (CE) with postcolumn laser-induced fluorescence detection (LIF) was used to analyze single skeletal muscle fibers from young and old rats. Due to selective labeling of mitochondria with 10-N-nonyl acridine orange, the zeptomole (10(-21) mole) sensitivity, and the high separation power, three properties of individual mitochondrial particles were revealed: the number, the distributions of cardiolipin, and their electrophoretic mobilities. Type I fibers had more mitochondrial particles and cardiolipin per particle than did type IIb fibers from rats of similar age. Individual fibers of the same fiber type from young rats contained more mitochondrial particles and cardiolipin per particle than did fibers from old rats. There were fiber type-specific and age-specific differences in the electrophoretic mobility of individual mitochondrial particles. The CE-LIF results of individual mitochondrial particles are the first of their kind in that they reveal fiber type-specific and age-specific differences that are not obviously noticed in bulk measurements of heterogeneous tissues.
采用柱后激光诱导荧光检测（LIF）的毛细管电泳（CE）技术，对年轻和老年大鼠的单个骨骼肌纤维进行分析。由于用10-N-壬基吖啶橙对线粒体进行了选择性标记，检测灵敏度达到zeptomole（10⁻²¹摩尔），且分离能力强，从而揭示了单个线粒体颗粒的三个特性：数量、心磷脂分布及其电泳迁移率。在相似年龄的大鼠中，I型纤维比IIb型纤维含有更多的线粒体颗粒，且每个颗粒中的心磷脂含量更高。年轻大鼠同一纤维类型的单个纤维比老年大鼠的纤维含有更多的线粒体颗粒，且每个颗粒中的心磷脂含量更高。单个线粒体颗粒的电泳迁移率存在纤维类型特异性和年龄特异性差异。单个线粒体颗粒的CE-LIF结果尚属首次，因为它们揭示了在异质组织的整体测量中未明显注意到的纤维类型特异性和年龄特异性差异。
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