Attallah A M, Helmi H, el-Helali E, el-Mohamadi H
Office of Biologic Research and Review, National Institutes of Health, Bethesda, MD.
Cancer Detect Prev. 1991;15(6):495-9.
A mouse monoclonal antibody (CK1K10) specific for keratin was developed. Enzyme immunoassays (EIAs) were performed to detect keratin in urine samples using CK1K10 and anti-mouse IgG. A high degree of sensitivity and specificity was obtained in the detection of bladder cancer patients. A fast dot-ELISA was developed in which urine is applied onto nitrocellulose (NC) sheets or dipsticks. Dipsticks coated with urine samples can be stored at room temperature for more than 5 days and at -20 degrees C for more than 3 months without loss of efficiency. A high positive correlation was found between the fast dot-ELISA and other EIAs. The assay does not require sophisticated equipment nor highly trained technical staff and can be performed in less than 30 min. The keratin dot-ELISA could be a reliable screening test for the early detection of bladder cancer.
开发了一种针对角蛋白的小鼠单克隆抗体（CK1K10）。使用CK1K10和抗小鼠IgG进行酶免疫测定（EIA）以检测尿液样本中的角蛋白。在检测膀胱癌患者时获得了高度的敏感性和特异性。开发了一种快速斑点酶联免疫吸附测定法，即将尿液应用于硝酸纤维素（NC）片或试纸条上。涂有尿液样本的试纸条可在室温下保存5天以上，在-20℃下保存3个月以上而不会失去效力。发现快速斑点酶联免疫吸附测定法与其他酶免疫测定法之间存在高度正相关。该测定法不需要复杂的设备，也不需要训练有素的技术人员，并且可以在不到30分钟内完成。角蛋白斑点酶联免疫吸附测定法可能是一种用于早期检测膀胱癌的可靠筛查试验。
