[米托蒽醌脂质体的制备及其经皮给药后在大鼠体内的分布研究]
[Preparation of mitoxantrone liposomes and study on its in vivo distribution in rats after transdermal delivery].
作者信息
Chen Tong, Hou Shi-Xiang, Sun Yi-Yi, Zheng Yu
机构信息
Department of Pharmaceutics Sciences, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 Nov;37(6):934-7.
OBJECTIVE
To investigate in vivo distribution of the mitoxantrone(MIT) liposomes in SD rats after transdermal delivery.
METHODS
The mitoxantrone liposomes were prepared by thin-film method. Particle size, particle distribution and delta-potential of colloid solution were obtained on laser scatterometer; the encapsulation efficiency was measured by membrane diffusion technique. The time courses of mitoxantrone concentration in vivo after the transdermal delivery of mitoxantrone liposomes were measured by HPLC assays and compared with the injection of mitoxantrone solution.
RESULTS
The mean diameter of the MIT liposomes was 50.98 nm, with the entrapping efficiency of 100%. The liposomes had perfect shape. A significantly higher amount of mitoxantrone was delivered in cutis after transdermal delivery of mitoxantrone liposomes, compared with the injection of mitoxantrone solution; meanwhile, a significantly lower amount of mitoxantrone was delivered in plasma and other tissues after transdermal delivery of mitoxantrone liposomes, compared with the injection of mitoxantrone solution.
CONCLUSION
Transdermal delivery of mitoxantrone liposomes could be a potential therapy for cutaneous malignant melanoma.
目的
研究米托蒽醌（MIT）脂质体经皮给药后在SD大鼠体内的分布情况。
方法
采用薄膜法制备米托蒽醌脂质体。用激光散射仪测定胶体溶液的粒径、粒度分布和δ电位；采用膜扩散技术测定包封率。通过高效液相色谱法测定米托蒽醌脂质体经皮给药后体内米托蒽醌浓度的时间进程，并与米托蒽醌溶液注射组进行比较。
结果
MIT脂质体的平均直径为50.98 nm，包封率为100%。脂质体形状完美。与米托蒽醌溶液注射组相比，米托蒽醌脂质体经皮给药后皮肤中递送的米托蒽醌量显著更高；同时，与米托蒽醌溶液注射组相比，米托蒽醌脂质体经皮给药后血浆和其他组织中递送的米托蒽醌量显著更低。
结论
米托蒽醌脂质体经皮给药可能是皮肤恶性黑色素瘤的一种潜在治疗方法。
Zotero 插件