脂质体包裹硫酸长春新碱和盐酸米托蒽醌的制备
[Preparation of liposome entrapped vincristine sulfate and mitoxantrone chlorhydric acid].
作者信息
Chen Tong, Hou Shi-xiang, Wang Yong-yan, Zhang Wen-sheng, Shi Bi-qiong
机构信息
Institute of Resources Medicine and Chinese Medical Resources, Beijing Normal University, Beijing 100875, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2007 Apr;32(8):678-81.
OBJECTIVE
To investigate the prepation of the liposome carried with vincristine sulfate (VCR) and mitoxantrone chlorhydric acid (MTO) and to evaluate the quality of the liposome.
METHOD
The liposome carried with VCR and MIT was prepared by pH-gradients method and reverse evaporation technique. HPLC was employed to determine VCR and MIT entrapping efficiency of liposomal. Laser particle analyzer was applied to determine the size and zeta potential of the liposomes carried with VCR and MTO.
RESULT
The mean diameter of the liposome carried with MIT and VCR was 72.22 nm, with the entrapping rate of 95.77% for VCR and 99.53% for MTO. The liposome had perfect shape.
CONCLUSION
The liposomes with high entrapping rate and small particle size had been prepared by pH-gradient method and reverse evaporation technique.
目的
研究硫酸长春新碱（VCR）和盐酸米托蒽醌（MTO）脂质体的制备方法，并评价脂质体的质量。
方法
采用pH梯度法和逆相蒸发技术制备VCR和MTO脂质体。采用高效液相色谱法测定脂质体中VCR和MTO的包封率。应用激光粒度分析仪测定VCR和MTO脂质体的粒径和ζ电位。
结果
VCR和MTO脂质体的平均粒径为72.22nm，VCR包封率为95.77%，MTO包封率为99.53%。脂质体形态良好。
结论
采用pH梯度法和逆相蒸发技术制备了包封率高、粒径小的脂质体。
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