Celardo A, Dell'Elba G, Frassanito R, Benfenati E, Bonati M
Istituto di Ricerche Farmacologiche Mario Negri, Consorzio Mario Negri Sud, S. Maria Imbaro, Italy.
J Chromatogr. 1991 Aug 23;568(2):407-18. doi: 10.1016/0378-4347(91)80178-f.
A sensitive high-performance liquid chromatographic assay for isbufylline and its major metabolites in rabbit blood and urine is described. After extraction, samples were eluted by a linear reversed-phase gradient. Specimens obtained after intravenous administration of isbufylline to rabbits were analysed to identify and subsequently quantify the potential metabolites. Using the ultraviolet absorption trace on the recorder as a reference, elution fractions were collected and analysed by mass spectrometry with the direct inlet system and gas chromatography-mass spectrometry after derivatization. Seven metabolites were identified and another five quantified. The method is specific, accurate, reproducible and recommended for pharmacokinetic studies.
本文描述了一种用于测定兔血和尿中异布福林及其主要代谢物的灵敏高效液相色谱法。萃取后，样品通过线性反相梯度洗脱。对静脉注射异布福林给兔后获得的样本进行分析，以鉴定并随后定量潜在的代谢物。以记录仪上的紫外吸收痕量为参照，收集洗脱级分，并通过直接进样系统的质谱法以及衍生化后的气相色谱-质谱法进行分析。鉴定出7种代谢物，另外5种进行了定量。该方法具有特异性、准确性、可重复性，推荐用于药代动力学研究。
