Barter L S, Mark L O, Smith A C, Antognini J F
Department of Anesthesiology and Pain Medicine, University of California Davis, California, USA.
Vet Res Commun. 2007 Aug;31(6):757-63. doi: 10.1007/s11259-007-0077-5. Epub 2007 Jan 19.
Amphibians are commonly used in biomedical research, including studies of mechanisms of anaesthetic action. There is, however, little published work describing the kinetics of inhaled anaesthetic agents or the potency of isoflurane in amphibians. Ten Northern leopard frogs were exposed to a constant isoflurane concentration of 1.0%, 1.2% or 1.5% atm for 4 h, and their response to a noxious stimulus was tested every 20 min. Each frog was anaesthetized with each concentration in random order and allowed at least 16 h to recover between anaesthetic exposures. Frogs were then pithed and the protocol was repeated. Frogs first displayed immobility during stimulus application at 80 min, and the proportion of animals becoming immobile steadily increased to reach a stable level at 4 h. The 50% effective dose for isoflurane in intact and pithed frogs did not differ, and was 1.15 and 1.25% atm, respectively. The potency of isoflurane in leopard frogs was similar to that reported in mammalian species. Cutaneous uptake of anaesthetic is effective given sufficient time, approximately 4 h in this study. Forebrain structures appear to be unimportant for the immobilizing action of isoflurane in the frog.
两栖动物常用于生物医学研究，包括麻醉作用机制的研究。然而，关于两栖动物吸入麻醉剂的动力学或异氟烷效力的已发表研究很少。将10只北美豹蛙暴露于1.0%、1.2%或1.5% atm的恒定异氟烷浓度下4小时，每20分钟测试它们对有害刺激的反应。每只青蛙以随机顺序用每种浓度进行麻醉，并且在两次麻醉暴露之间至少允许16小时恢复。然后将青蛙处死并重复该方案。青蛙在刺激施加80分钟时首次表现出不动，并且不动的动物比例稳步增加，在4小时时达到稳定水平。完整青蛙和处死青蛙的异氟烷半数有效剂量没有差异，分别为1.15% atm和1.25% atm。异氟烷在豹蛙中的效力与在哺乳动物物种中报道的相似。在有足够时间的情况下，麻醉剂的皮肤吸收是有效的，在本研究中约为4小时。前脑结构对于异氟烷在青蛙中的制动作用似乎并不重要。
