Melamed N, Rahamimoff R
Department of Physiology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
J Basic Clin Physiol Pharmacol. 1991 Jan-Jun;2(1-2):63-85. doi: 10.1515/jbcpp.1991.2.1-2.63.
Confocal imaging was performed on the ceratomandibularis nerve muscle preparation of the lizard Anolis carolinensis, using 4-Di-2-ASP as a fluorescent probe. The imaging system consisted of a Sarastro Phoibos 1000 (Molecular Dynamics) scanning system and a Zeiss Universal microscope. The data were analyzed using the VANIS set of programs on a Silicon Graphics Personal Iris computer. A three dimensional reconstruction of the nerve terminals was performed using look-through and depth-coded projections. The volume of the nerve terminal was estimated in 15 neuromuscular junctions and found to be 84,835 microns 3 (+/- 8558 SEM). The largest diameter in each one of the 178 individual boutons was estimated from the look-through projections of 17 nerve terminals in 9 preparations. It was found to be 4.71 microns (+/- 0.08 SEM). The diameter perpendicular to the largest diameter in the same projection at 0 degrees was 3.3 microns (+/- 0.054 SEM). Thus it seems that the synaptic boutons of the ceratomandibularis are suitable for combined optical and electrophysiological recordings.
使用4-Di-2-ASP作为荧光探针，对卡罗来纳安乐蜥的角颌神经肌肉标本进行共聚焦成像。成像系统由Sarastro Phoibos 1000（分子动力学）扫描系统和蔡司万能显微镜组成。数据在硅图形个人虹膜计算机上使用VANIS程序集进行分析。使用透视和深度编码投影对神经末梢进行三维重建。在15个神经肌肉接头处估计神经末梢的体积，发现为84,835立方微米（±8558标准误）。从9个标本中17个神经末梢的透视投影估计178个单个终扣中每个终扣的最大直径。发现其为4.71微米（±0.08标准误）。在同一投影中与最大直径垂直的0度处的直径为3.3微米（±0.054标准误）。因此，角颌肌的突触终扣似乎适合进行光学和电生理联合记录。
