Horsburgh K, McCulloch J
Wellcome Surgical Institute, University of Glasgow, U.K.
Brain Res. 1991 Nov 1;563(1-2):306-10. doi: 10.1016/0006-8993(91)91552-c.
Quantitative autoradiography of [3H]forskolin and [3H]phorbol 12,13 dibutyrate (PDBu) binding was examined 21 days after unilateral lesioning of the rat visual cortex using ibotenic acid. In the same animals, the functional deficit was assessed using quantitative [14C]-2-deoxyglucose autoradiography. [3H]Forskolin binding was significantly reduced in each layer of the lesioned visual cortex by at least 40% compared to the contralateral hemisphere. Significant reductions in [3H]forskolin binding were observed in the superior colliculus (-15%) and dorsal lateral geniculate body (-12%) ipsilateral to the lesioned cortex. [3H]PDBu binding was significantly reduced in the lesioned visual cortex (layers V-VI) by 34%, compared with the control hemisphere. There were no significant alterations in [3H]DPBu binding in any other brain regions. Following ibotenate-induced lesioning of the visual cortex, glucose use was significantly reduced throughout the lesioned cortex by at least 25% with minor alterations in glucose use in the ipsilateral dorsal lateral geniculate body and superior colliculus. The present study highlights the relative robustness of [3H]PDBu binding compared to [3H]forskolin binding after excitotoxic damage to the cerebral cortex and suggests that [3H]forskolin binding sites are present on cortical efferent fibres.
在使用鹅膏蕈氨酸对大鼠视觉皮层进行单侧损伤21天后，检测了[3H]福斯高林和[3H]佛波酯12,13 -二丁酸酯（PDBu）结合的定量放射自显影。在同一批动物中，使用定量[14C]-2-脱氧葡萄糖放射自显影评估功能缺陷。与对侧半球相比，损伤的视觉皮层各层中[3H]福斯高林结合显著减少至少40%。在损伤皮层同侧的上丘（-15%）和背外侧膝状体（-12%）中观察到[3H]福斯高林结合显著减少。与对照半球相比，损伤的视觉皮层（V - VI层）中[3H]PDBu结合显著减少34%。在任何其他脑区，[3H]DPBu结合均无显著改变。在鹅膏蕈氨酸诱导的视觉皮层损伤后，损伤皮层各处的葡萄糖利用显著减少至少25%，同侧背外侧膝状体和上丘的葡萄糖利用有轻微改变。本研究强调了在大脑皮层兴奋性毒性损伤后，与[3H]福斯高林结合相比，[3H]PDBu结合的相对稳定性，并表明[3H]福斯高林结合位点存在于皮层传出纤维上。
