Chalmers D T, McCulloch J
Wellcome Neuroscience Group, Wellcome Surgical Institute, University of Glasgow, U.K.
Neurosci Lett. 1989 Feb 27;97(3):298-304. doi: 10.1016/0304-3940(89)90614-9.
Glutamate is a major neurotransmitter in the rat visual system. The effect of lesion-induced functional deficit on [3H]glutamate binding sites within anatomical components of the visual pathway has been examined using quantitative autoradiography. In the same animals, the magnitude and extent of the functional deficit was assessed with quantitative [14C]-2-deoxyglucose autoradiography. At 24 h after orbital enucleation, significant reductions (approx. 25%) in glutamate binding were present throughout the visual cortex but there were no significant alterations in glutamate binding in the two principal projections of the retina, the superior colliculus or lateral geniculate body. Function-related glucose use was significantly reduced throughout the visual pathway after orbital enucleation. Thus, alterations in the number of glutamate binding sites occur in some, but not all, of the regions in a polysynaptic pathway in which activity is altered.
谷氨酸是大鼠视觉系统中的一种主要神经递质。利用定量放射自显影技术，研究了损伤诱导的功能缺陷对视觉通路解剖结构中[3H]谷氨酸结合位点的影响。在同一批动物中，用定量[14C]-2-脱氧葡萄糖放射自显影评估功能缺陷的程度和范围。眼球摘除术后24小时，整个视觉皮层的谷氨酸结合显著减少（约25%），但视网膜的两个主要投射部位，即上丘或外侧膝状体的谷氨酸结合没有显著改变。眼球摘除术后，整个视觉通路中与功能相关的葡萄糖利用显著减少。因此，谷氨酸结合位点数量的改变发生在多突触通路中一些但并非全部活性发生改变的区域。
