新型含PGM的不完全赛比克佐剂在兔体内的有效性。

Effectiveness of novel PGM-containing incomplete Seppic adjuvants in rabbits.

作者信息

Halassy Beata, Vdović Valerija, Habjanec Lidija, Balija Maja Lang, Gebauer Branka, Sabioncello Ante, Santek Tomislav, Tomasić Jelka

机构信息

Research and Development Department, Institute of Immunology, Inc., Rockefellerova 10, HR-10000 Zagreb, Croatia.

出版信息

Vaccine. 2007 Apr 30;25(17):3475-81. doi: 10.1016/j.vaccine.2006.12.040. Epub 2007 Jan 3.

DOI:10.1016/j.vaccine.2006.12.040

PMID:17239503

Abstract

Peptidoglycan monomer (PGM) is adjuvant active molecule in experimental mice, although its adjuvanticity is much lower in comparison to potent adjuvants. The novel adjuvant formulations were developed by incorporation of PGM into Montanide ISA 206 and Montanide ISA 720 adjuvants, with the aim to enhance its adjuvanticity by protecting it from the fast degradation and metabolic clearance. Adjuvanticity of the novel adjuvant formulations was tested in rabbits for induction of protein-specific antibodies. Both novel adjuvants ISA206(PGM) and ISA720(PGM) were significantly stronger than Montanide adjuvants themselves, and also significantly more potent than Complete Freund Adjuvant. Montanide ISA 720 was shown as much better carrier of PGM, since the novel ISA720(PGM) adjuvant was significantly stronger adjuvant than the ISA206(PGM).

摘要

肽聚糖单体（PGM）在实验小鼠中是一种佐剂活性分子，尽管与强效佐剂相比其佐剂活性要低得多。通过将PGM掺入Montanide ISA 206和Montanide ISA 720佐剂中开发了新型佐剂配方，目的是通过保护其免受快速降解和代谢清除来增强其佐剂活性。在兔子中测试了新型佐剂配方诱导蛋白质特异性抗体的佐剂活性。新型佐剂ISA206（PGM）和ISA720（PGM）都比Montanide佐剂本身显著更强，并且也比完全弗氏佐剂显著更有效。Montanide ISA 720被证明是PGM的更好载体，因为新型ISA720（PGM）佐剂比ISA206（PGM）是显著更强的佐剂。