Courtin Francois, Huerre Michel, Fyfe Janet, Dumas Paul, Boschiroli Maria L
Veterinary Clinic, 03 Marcillat, France.
J Feline Med Surg. 2007 Jun;9(3):238-41. doi: 10.1016/j.jfms.2006.11.007. Epub 2007 Jan 17.
A 2-year-old, 4 kg, healthy, domestic shorthair female cat presented with ulcerated subcutaneous nodules on the commissures of its mouth. The cat was negative for feline leukaemia virus and feline immunodeficiency virus. Skin mycobacteriosis was diagnosed after detection of numerous acid-fast bacilli in Ziehl Neelsen-stained smears from the ulcers. Feline leprosy was suspected following preliminary polymerase chain reaction results: positive for Mycobacterium genus but negative for Mycobacterium tuberculosis and Mycobacterium avium complexes. Mycobacterium lepraemurium was later identified following DNA sequence analysis of the 5' end of the 16S rRNA gene and the 16S-23S internal transcribed spacer region. Microscopic lesions consisted of pyogranulomas containing mainly large foamy macrophages with 10-100 intra-cellular acid-fast bacilli per field. The cat was cured after surgery and a 14-week course of clofazimine (30 mg daily) and clarithromycin (50 mg twice daily).
一只2岁、体重4千克、健康的家养短毛雌性猫，嘴角出现溃疡性皮下结节。该猫的猫白血病病毒和猫免疫缺陷病毒检测均为阴性。在对溃疡处进行齐-尼氏染色涂片检测发现大量抗酸杆菌后，诊断为皮肤分枝杆菌病。根据初步聚合酶链反应结果怀疑为猫麻风病：分枝杆菌属阳性，但结核分枝杆菌和鸟分枝杆菌复合体阴性。后来通过对16S rRNA基因5'端和16S - 23S内转录间隔区进行DNA序列分析，鉴定为鼠麻风分枝杆菌。显微镜下病变为脓性肉芽肿，主要由大的泡沫状巨噬细胞组成，每个视野中有10 - 100个细胞内抗酸杆菌。这只猫在接受手术以及14周的氯法齐明（每日30毫克）和克拉霉素（每日50毫克，分两次服用）治疗后痊愈。
